T84 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88021101, human colon (lung metastasis), Epithelial

• Synonim(y): T-84 Cells
• UNSPSC Code: 41106514
• Biological source: human colon (lung metastasis)
• Relevant disease(s): metastasis
• Growth mode: Adherent
• Karyotype: 2n = 46, modal no. 56
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Właściwości

• biological source: human colon (lung metastasis)
• description: Human colon carcinoma
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 88021101-DNA-5UG, pkg of vial of cells 88021101-1VL
• growth mode: Adherent
• karyotype: 2n = 46, modal no. 56
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Opis

Application

Tumourigenicity, neurotransmitter and hormone studies

Human T84 cell line has been used for comparative genome analysis of Salmonella enterica serovar Typhimurium definitive type 2 (DT2) isolate 94-213 and S. Typhimurium isolates SL1344, DT104 and D23580. The ability of these isolates to invade culture medium has also been studied. It has also been used to construct and study the suitability of an in vitro model to study chemotherapy-induced mucosal damage.

Biochem/physiol Actions

Derived from a lung metastasis of colon carcinoma in a 72 year old male. Tumour tissue was inoculated sub-cutaneously and serially transplanted in BALB/c nude mice and subsequently established in in vitro culture. The histological characteristics of the tumour were maintained throughout the transplantation procedure. They have receptors for a range of peptide hormones and neurotransmitters. The cells grow as monolayers, exhibit tight junctions and desmosomes between adjacent cells.

Human colon carcinoma

Preparation Note

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 - 1:4 i.e. seeding at 1-3 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells grow slowly and may not form a complete monolayer, maintain at high density with a minimum of 25% confluency.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

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