MDCK-SIAT1 Cell Line canine

5071502, canine kidney, Epithelial

• Synonim(y): MDCK-SIAT1 Cells
• Kod UNSPSC: 41106514

Właściwości

• product name: MDCK-SIAT1 Cell Line canine, 05071502
• pochodzenie biologiczne: canine kidney
• opis: Canine Cocker Spaniel Kidney Sialic Acid Over Expression
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Epithelial
• produkty: Not specified
• receptory: Enhanced expression of 6-linked sialic acids
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Canine Cocker Spaniel Kidney Sialic Acid Over Expression

Opis linii komórkowej

This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold?higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-α2,6-galactose moieties.

pożywka hodowlana

DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

Inne uwagi

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