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C-44010

Lymphocyte Separation Medium 1077

500 ml

Synonim(y):

Lymphocyte Medium, Separation Medium for Lymphocytes

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Do Państwa/SKUDostępnośćCena netto
500 mL
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534,00 zł

Informacje o tej pozycji

NACRES:
NA.71
UNSPSC Code:
12352207

534,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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form

aqueous solution

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

storage temp.

room temp

General description

Lymphocyte Separation Medium 1077

Application

Lymphocyte Separation Medium 1077 is intended for the separation of the portion of vital mononuclear cells from whole blood, buffy coats, bone marrow and several other starting materials, e.g. crude cell preparations, by means of low density gradient centrifugation. Lymphocyte separation Medium has a density of 1.077 g/ml at 20°C. It is sterile, ready-to use and based on Ficoll 400 and sodium diatrizoate providing optimal physiological parameters and low cytotoxicity.

Preparation Note

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Lymphocyte Separation Medium 1077 is light-sensitive! Store the medium at room temperature in the dark immediately after arrival. Do not freeze. If stored properly, the product is stable until the expiry date stated on the label.

Analysis Note

All lots of PromoCell Lymphocyte Separation Medium 1077 are subjected to comprehensive quality control tests. Each lot is routinely tested for function and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria).
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Ta pozycja
CN151D-250C-28030R813D-250
Lymphocyte Separation Medium 1077 500 ml

Sigma-Aldrich

C-44010

Lymphocyte Separation Medium 1077

Pożywka do różnicowania komórek mięśni szkieletowych psów (250 ml)

Sigma-Aldrich

CN151D-250

Pożywka do różnicowania komórek mięśni szkieletowych psów (250 ml)

Pożywka dla komórek jednojądrzastych Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

Sigma-Aldrich

C-28030

Pożywka dla komórek jednojądrzastych

Podłoże do różnicowania neuronalnych komórek macierzystych szczura (250 ml)

Sigma-Aldrich

R813D-250

Podłoże do różnicowania neuronalnych komórek macierzystych szczura (250 ml)

form

aqueous solution

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

packaging

pkg of 500 ml

packaging

-

packaging

pkg of 500 ml

packaging

-

shipped in

ambient

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

room temp

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

pictograms

Corrosion
GHS05

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Klasa składowania

12 - Non Combustible Liquids

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  

  1. What's the procedure of lymphocytes separation with this media and the quantity used .

    1 answer

    1. This is a product of PromoCell. Please see the instructions for use provided by the manufacturer:
      1. Fill Lymphocyte Separation Medium 1077 in a tube. Use 3 ml for a 15 ml tube and 20 ml for a 50 ml tube.
      2. Hold the tube at a 45° angle and carefully load the cell suspension to be separated on top of the separation medium.
      Note: Perform this step slowly but constantly. Take care that the two phases do not mix and a sharp border is present.
      For best results, use an electrical pipet aid with EX-function allowing the slow and constant efflux of the cell suspension from the pipet.
      3. Centrifuge the tube at 440 x g for 40 minutes at room temperature without brake.
      4. Carefully aspirate most of the supernatant without disturbing the layer of mononuclear cells in the interphase.
      5. Aspirate the ring of mononuclear cells from the interphase using a pipet. Keep the volume as small as possible.
      6. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 360 x g for 10 minutes.
      7. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 200 x g for 10 minutes X 2
      8. The mononuclear cell preparation is ready.

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  2. What percentage of lymphocytes/monocytes, etc. would be expected if they use it on fresh human blood?

    1 answer

    1. When utilizing the Lymphocyte Separation Medium 1077 (cat. #C-44010) on fresh human whole blood, the expected percentage of lymphocytes/monocytes typical for this sample is usually in the range of 70-90% of the enriched PBMCs.

      Helpful?

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