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Merck

C-28057

Sigma-Aldrich

Macrophage Base Medium DXF

Basal Medium, 250 ml

Synonim(y):

Macrophage Culture Medium

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.71

Postać

liquid

opakowanie

pkg of 250 ml

metody

cell culture | mammalian: suitable

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Opis ogólny

Macrophage Base Medium DXF (supplements sold separately)

Zastosowanie

The Macrophage Base Medium DXF is the user-customizable version of the Macrophage Generation Media DXF that does not include cytokines. It must be adequately supplemented by the user for the generation of monocyte-derived Macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. In the latter case, the Monocyte Attachment Medium (C-28051) is also needed. Recommended for:Human Mononuclear Cells (hMNC), freshly isolatedHuman Monocytes (hMo), freshly isolated

Komponenty

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Jakość

All lots of PromoCell Macrophage Media DXF are subjected to comprehensive quality control tests using human peripheral blood mononuclear cells. Each lot of PromoCell Macrophage Media DXF is tested for the ability to support Macrophage differentiation verified by morphological evaluation and flow cytometry analysis. Each lot of PromoCell Monocyte Attachment Medium is tested for its ability to support optimal attachment and viability of peripheral blood Monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Przechowywanie i stabilność

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Complete Medium supplemented with cytokines should be used within 2 weeks. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Rutyna subkultury

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Kod klasy składowania

10 - Combustible liquids


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Produkty

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Odkryj ponad 350 produktów PromoCell na Sigma.com, w tym kriokonserwowane komórki krwi i pożywki do hodowli komórkowych dla różnych komórek ludzkich.

Protokoły

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

Generowanie fagocytarnych makrofagów M1 i M2 z ludzkich PBMC w ciągu 10 dni w pożywkach do hodowli komórkowych niezawierających surowicy i ksenonów. Poznaj ponad 350 produktów PromoCell.

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