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C0543

Mouse Collagen Type IV

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation

Synonim(y):

Type IV collagen, EHS-collagen

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Do Państwa/SKUDostępnośćCena netto
1 vial
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1490,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352202
NACRES:
NA.75
EC Number:
232-697-4
MDL number:
Biological source:
mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable

1490,00 zł


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Nazwa produktu

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

biological source

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

Quality Segment

type

Type IV (Miller)

product line

BioReagent

form

lyophilized powder

packaging

glass bottle of 1 vial

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

solubility

0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

General description

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.

Application

Recommended for use as a cell culture substratum at 6-10 μg/cm2.
Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:
  • in adhesion assays.
  • in hydrogel fabrication.
  • to coat cell culture plates for cell differentiation.
  • to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.

Biochem/physiol Actions

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Preparation Note

0.75 mg/vial
Chloroform treated.
Pepsin is not used in the preparation.

Other Notes

Native collagen
We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.
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Ta pozycja
C6745C3867C7661
biological source

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

biological source

human cell culture

biological source

rat tail

biological source

rat tail

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

solution

form

liquid

form

powder

solubility

0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)

solubility

-

solubility

soluble, clear to hazy, colorless (solubilized in 0.02M acetic acid)

solubility

soluble

Gene Information

mouse ... Col4a1(12826), Col4a2(12827), Col4a3(12828), Col4a5(12830), Col4a6(94216)

Gene Information

human ... COL4A1(1282)

Gene Information

rat ... Col1a1(29393)

Gene Information

rat ... Col1a1(29393)


Klasa składowania

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

  1. Could you provide me with the observed molecular weight of the Rat Collagen Type IV product from the Engelbreth-Holm-Swarm murine sarcoma basement membrane? CAS Number:9007-34-5

    1 answer
    1. The molecular weight for this product has not been determined in-house. Other sources have reported the molecular weight to be 484.7 g/mol.

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