B4554

BES

BioPerformance Certified, suitable for cell culture, ≥99.0%

• Synonim(y): N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
• Wzór empiryczny (zapis Hilla): C₆H₁₅NO₅S
• Numer CAS: 10191-18-1
• Masa cząsteczkowa: 213.25
• Beilstein: 1781572
• Numer WE: 233-465-5
• Numer MDL: MFCD00007533
• Kod UNSPSC: 12161700
• Identyfikator substancji w PubChem: 24891788
• NACRES: NA.25

Właściwości

• klasa czystości: BioPerformance Certified
• Próba: ≥99.0%
• Postać: crystalline
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: endotoxin and bioburden, tested
• pH: 2.5-5.0 (25 °C, 213.3 g/L)
• przydatny zakres pH: 6.4-7.8
• pKa (25°C): 7.1
• rozpuszczalność: water: 0.5 g/mL, clear, colorless
• Zastosowanie: diagnostic assay manufacturing
• obecność zanieczyszczeń: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
• temp. przechowywania: room temp
• ciąg SMILES: OCCN(CCO)CCS(O)(=O)=O
• InChI: 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
• Klucz InChI: AJTVSSFTXWNIRG-UHFFFAOYSA-N

Opis

Opis ogólny

N-N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) is a zwitterionic sodium sulphate buffer used in biochemistry and molecular biology research. BES buffer used as a secondary pH standard can help maintain a stable pH range in solutions, particularly in the range of 6.2-7.6 pH range of 6.6 to 7.4. It ​is used in a study to evaluate that buffers minimize the action of interfering factors in the bacterial endotoxins test. The application of BES as a buffer solution for calcium phosphate-mediated transfection of eukaryotic cells with plasmid DNA has also been studied.

Zastosowanie

BES buffer has been used:

• as a supplement in opti-minimal essential medium (MEM) culture media to culture thymocytes for In vitro analysis of thymocyte apoptosis
• as a component of borate buffer saline (BBS) for adeno-associated virus (AAV) vector production, extraction, and purification
• as a component of diluent one for semen collection and cryopreservation

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves