B0561

Monoclonal Anti-BUB1 antibody produced in mouse

2 mg/mL, clone 14H5, purified immunoglobulin, buffered aqueous solution

• Synonim(y): Anti-Budding Uninhibited By Benzimidazoles 1
• UNSPSC Code: 12352203
• NACRES: NA.44
• MDL number: MFCD04040371
• Conjugate: unconjugated
• Clone: 14H5, monoclonal
• Application: ARR, ELISA (i), ICC, IP, WB
• Citations: 6

Właściwości

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 14H5, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: 2 mg/mL
• technique(s): immunocytochemistry: 4-8 μg/mL using HeLa cells treated with 20 μg/mL nacodazol, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: suitable
• isotype: IgG1
• UniProt accession no.: O43683
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... BUB1(699)

Opis

General description

BUB1 is spindle checkpoint kinase that modulates kinetochore-independent segregation of chromosomes and cell proliferation. This kinase regulates the phosphorylation of MAD1 and itself. As a result, BUB1 mediates chromosome-spindle attachments during mitosis. BUB1 also phosphorylates Cdc20 which subsequently facilitates accurate checkpoint signaling . Monoclonal Anti-BUB1 antibody is specific for human BUB1 (approx. 150 kDa).

Immunogen

recombinant human BUB1.

Application

Monoclonal Anti-BUB1 antibody is suitable for use in western blot, indirect ELISA, microarray, immunocytochemistry (4-8 μg/mL using HeLa cells treated with 20 μg/mL nacodazol) and immunoprecipitation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable