This enzyme is soluble in aqueous buffers and water, at 20 mg/mL. This material may also be prepared 100 mM Tris HCl buffer, pH 8.0, containing 0.02% BSA (0.3 mg/ml), yielding a clear, colorless solution.
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A9770
Aldehyde Dehydrogenase, potassium-activated from yeast
lyophilized powder, ≥10 units/mg protein
Synonim(y):
ALDH, CoA-independent aldehyde dehydrogenase, m-methylbenzaldehyde dehydrogenase, Aldehyde:NAD[P]+ oxidoreductase
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
25 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 415,00 zł |
100 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1340,00 zł |
250 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2470,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-846-3
MDL number:
Specific activity:
≥10 units/mg protein
Biological source:
yeast
415,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
yeast
Quality Segment
form
lyophilized powder
specific activity
≥10 units/mg protein
storage condition
(Tightly closed. Dry)
color
white
UniProt accession no.
foreign activity
NADH oxidase ≤0.01%, NADPH oxidase ≤0.01%, alcohol dehydrogenase ≤0.01%, lactic dehydrogenase ≤0.01%
storage temp.
2-8°C
General description
Aldehyde dehydrogenase, from yeast, is used to catalyze the oxidation of a wide range of substrates, such as acetaldehyde, formaldehyde, propionaldehyde, n-butylaldehyde, isobutylaldehyde, n-valeraldehyde, caproaldehyde, benzaldehyde, glycoaldehyde, D-glyceraldehyde, malonic semialdehyde, and succinic aldehyde. Aldehyde dehydrogenase, from yeast has been used to study the production of ethanol and isobutanol. Aldehyde dehydrogenase, from Sigma, has been used along with alcohol dehydrogenase to measure ethanol production during the characterization of glycolytic metabolism and ion transport in Candida albicans.
The family of aldehyde dehydrogenase (ALDH) contains 19 genes in humans. It is localized in the nucleus, cytosol, mitochondria and endoplasmic reticulum of the cell.
Research area: Cell Signaling
Research area: Cell Signaling
Application
Aldehyde Dehydrogenase, potassium-activated from yeast has been used:
- for enzyme immobilization and to oxidize formaldehyde to formate
- to study the functional relation between hydrazone
- to measure ethanol production[1].
Biochem/physiol Actions
Aldehyde dehydrogenase is a tetramer and has several different isoforms. Aldehyde dehydrogenase is inhibited by propylurea, crotonaldehyde, n-propyl isocyanate, cyclohexyl isocyanate, 1-n-propyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea, and 1-methyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea. The enzyme tested in 0.01 M pyrophosphate buffer shows a sharp optimum around pH 9.3 with acetaldehyde as substrate.[2]
Aldehyde dehydrogenase (ALDH) modulates the non-P450 aldehyde reduction enzyme system. It protects the cell from the effects of toxic aldehydes. Mutations in this gene leads to Sjogren‐Larsson syndrome, Larsson syndrome, type II hyperprolinemia and cancer. ALDH-2 lowers cardiac ischemia, which arises due to myocardial infarction or post cardiac surgery.Aldehyde Dehydrogenase (ALDH) participates in the functioning of regulatory T cells that are a part of the immune system. It is also involved in cellular detoxification, the amino acid metabolism and protects cells against ultraviolet (UV) rays-induced damage. ALDH plays a role in retinoic acid biosynthesis and signaling.[3]
Physical form
Contains potassium phosphate salts
Other Notes
One unit will oxidize 1 micromole of acetaldehyde to acetic acid per minute pH 8.0 at 25 deg C.
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Ta pozycja | |||
|---|---|---|---|
| specific activity ≥10 units/mg protein | specific activity ~20 units/mg protein (At 25 °C with acetaldehyde as the substrate.) | specific activity ≥2.0 units/mg protein | specific activity ≥19 units/mg protein |
| biological source yeast | biological source yeast | biological source - | biological source - |
| form lyophilized powder | form lyophilized | form lyophilized powder | form lyophilized solid |
| storage condition (Tightly closed. Dry) | storage condition - | storage condition - | storage condition OK to freeze |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. - | UniProt accession no. - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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how to dissolve this enzyme and which solvent to use. Please share the protocol
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of Aldehyde Dehydrogenase, potassium-activated from baker's yeast (S. cerevisiae), product A9770?
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Aldehyde dehydrogenase from bakers yeast, product A9770, is a tetramer consisting of subunits of 57 kDa. The molecular weights of its monomer, dimer, trimer, and tetramer are 57 kDa, 114 kDa, 171 kDa, and 228 kDa respectively. The monomers are thought to be assembled into tetramers in a heterologous square arrangement.
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