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A8811

Aldolase from rabbit muscle

ammonium sulfate suspension, 10-20 units/mg protein

Synonym(s):

D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase, Fructose-diphosphate Aldolase

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Size/SKUAvailabilityPrice
100 units
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$74.30
200 units
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$84.20
1000 units
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$327.00
5000 units
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$1,120.00

About This Item

CAS Number:
9024-52-6
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
4.1.2.13 (BRENDA, IUBMB)
MDL number:
MFCD00130453
Specific activity:
10-20 units/mg protein
Biological source:
rabbit muscle

$74.30

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biological source

rabbit muscle

Quality Segment

200

form

ammonium sulfate suspension

specific activity

10-20 units/mg protein

foreign activity

glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, lactic dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, pyruvate kinase ≤0.1%, triosephosphate isomerase ≤0.05%

storage temp.

2-8°C

General description

Aldolase exists as three isoforms in rabbit, which includes type A from muscle, type B from liver and brain associated type C. Aldolases correspond to a molecular weight of 158 kDa and exists as tetramer.

Application

Aldolase from rabbit muscle has been used:
  • in standard 1-phosphofructokinase from rabbit muscle (RPFK-1) assay
  • as a standard in the characterization of metabolic enzymes from glaucomatous tissues[1]
  • in fructose 2,6-bisphosphate assay of human cell lines[2]

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 [3].

Biochem/physiol Actions

Aldolase interaction with Wiskott-Aldrich syndrome protein (WASP) may modulate actin dynamics. It reverses the inhibition elicited by ascorbate on Muscle-type LDH (LDH-m4).
Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β 8 barrel fold [3].

Physical form

Crystalline suspension in 2.5 M (NH4)2SO4, 0.01 M Tris, pH 7.5, 0.001 M EDTA

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

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Aldolase from rabbit muscle ammonium sulfate suspension, 10-20 units/mg protein

Sigma-Aldrich

A8811

Aldolase from rabbit muscle

Aldolase from rabbit muscle lyophilized powder, ≥8.0 units/mg protein

Sigma-Aldrich

A2714

Aldolase from rabbit muscle

α-Glycerophosphate Dehydrogenase from rabbit muscle Type I, ammonium sulfate suspension, 100-300 units/mg protein

Sigma-Aldrich

G6751

α-Glycerophosphate Dehydrogenase from rabbit muscle

L-Lactic Dehydrogenase from rabbit muscle Type II, ammonium sulfate suspension, 800-1,200 units/mg protein

Sigma-Aldrich

L2500

L-Lactic Dehydrogenase from rabbit muscle

biological source

rabbit muscle

biological source

rabbit muscle

biological source

rabbit muscle

biological source

-

specific activity

10-20 units/mg protein

specific activity

≥8.0 units/mg protein

specific activity

100-300 units/mg protein

specific activity

800-1,200 units/mg protein

form

ammonium sulfate suspension

form

lyophilized powder

form

ammonium sulfate suspension

form

ammonium sulfate suspension

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

foreign activity

glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, triosephosphate isomerase ≤0.05%, lactic dehydrogenase ≤0.03%, pyruvate kinase ≤0.1%

foreign activity

glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, lactic dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, pyruvate kinase ≤0.1%, triosephosphate isomerase ≤0.05%

foreign activity

Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, Triosephosphate isomerase ≤0.02%

foreign activity

pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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