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A7058

Anti-polyHistidine−Peroxidase antibody, Mouse monoclonal

clone HIS-1, purified from hybridoma cell culture

Anti-polyHistidine−Peroxidase antibody, Mouse monoclonal

Synonim(y):

Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

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Do Państwa/SKUDostępnośćCena netto
1 vial
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4440,00 zł

Informacje o tej pozycji

NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Clone:
HIS-1, monoclonal
Application:
WB
Citations:
152

4440,00 zł


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biological source

mouse

Quality Segment

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HIS-1, monoclonal

form

lyophilized powder

packaging

vial of 0.5 mL

concentration

5-11 mg/mL

technique(s)

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

isotype

IgG2a

storage temp.

2-8°C

target post-translational modification

unmodified

General description

The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Immunogen

recombinant polyHistidine tagged fusion protein.

Application

Antibody suitable for immunoblotting. Working dilution 1:20



Also suitable for dot blot assays and ELISA

Biochem/physiol Actions

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
The antibody conjugate should be reconstituted with 0.5 ml deionized water.

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
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Ta pozycja
A5588SAB4200620H1029
conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

-

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

ascites fluid

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

HIS-1, monoclonal

clone

HIS-1, monoclonal

clone

HIS-1, monoclonal

clone

HIS-1, monoclonal

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

isotype

IgG2a

isotype

IgG2a

isotype

IgG2a

isotype

IgG2a


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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Klasa składowania

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Lot/Batch Number

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Questions

  1. To whom it may concern. Is the antibody Anti-his 6 tag hrseradish Peroxidase conjugated? Thank you

    1 answer
    1. This product is horseradish peroxidase conjugated.

      Helpful?

Reviews

Active Filters

  1. Ireland
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Very good

    Very good antibody, we use the antibody for wet lab practicals and it works very well.

    Helpful?