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Merck

A3813

Sigma-Aldrich

Anti-Human Kappa Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Synonim(y):

Goat Anti-Human Kappa Light Chains (Bound and Free)−AP

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About This Item

Numer MDL:
Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

goat

białko sprzężone

alkaline phosphatase conjugate

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

secondary antibodies

klon

polyclonal

Postać

buffered aqueous glycerol solution

reaktywność gatunkowa

human

metody

direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

Warunki transportu

wet ice

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... IGK@(50802)

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Opis ogólny

Human κ light chains are antibody fragments that regulate immunological responses in cells. Increased expression of κ light chains has been linked to chronic lymphocytic leukemia . The variable fragment of the κ light chain gene has also been associated with vaccine-induced antibody response to the polysaccharide coat in Haemophilus influenzae type b . Anti-Human κ Light Chains (Bound and Free-Alkaline Phosphatase antibody is specific for human κ light chain when tested against human IgA, IgG, IgM, IgGλ, IgAλ, IgMλ, Bence Jones κ and λ myeloma proteins.

Immunogen

Purified Bence Jones κ light chain

Zastosowanie

Anti-Human κ Light Chains (Bound and Free)-Alkaline Phosphatase antibody is suitable for use in ELISA. The product can also be used for dot blot (1:30,000) and immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Mouse-Human chimeric IgG′s specific for Pseudomonas aeruginosa serogroup 06 lipopolysaccharide were identified by ELISA in transfected CHO cell supernatants using Alkaline phosphatase conjugated goat anti-Human Kappa Light chains.

Postać fizyczna

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Thomas Bouquin et al.
Transgenic research, 11(2), 115-122 (2002-06-11)
Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus
Rogier A van Dijk et al.
Journal of lipid research, 53(12), 2773-2790 (2012-09-13)
The relationships between oxidation-specific epitopes (OSE) and lipoprotein (a) [Lp(a)] and progressive atherosclerosis and plaque rupture have not been determined. Coronary artery sections from sudden death victims and carotid endarterectomy specimens were immunostained for apoB-100, oxidized phospholipids (OxPL), apo(a), malondialdehyde-lysine
Sophie Vazulka et al.
ACS synthetic biology, 11(2), 820-834 (2022-01-19)
Antibody fragments such as Fab's require the formation of disulfide bonds to achieve a proper folding state. During their recombinant, periplasmic expression in Escherichia coli, oxidative folding is mediated by the DsbA/DsbB system in concert with ubiquinone. Thereby, overexpression of
L J Latorre-Rey et al.
Journal of thrombosis and haemostasis : JTH, 15(2), 341-355 (2016-12-09)
Essentials Platelet phenotypes can be modified by lentiviral transduction of hematopoietic stem cells. Megakaryocyte-specific lentiviral vectors were tested in vitro and in vivo for restricted expression. The glycoprotein 6 vector expressed almost exclusively in megakaryocytes. The platelet factor 4 vector
Michaela Strotbek et al.
Metabolic engineering, 20, 157-166 (2013-10-23)
MicroRNAs (miRNAs) are short non-coding RNAs that post-transcriptionally regulate the expression of different target genes and, thus, enable engineered gene networks to achieve complex phenotypic changes in mammalian cells. We hypothesized that exploiting this feature of miRNAs could improve therapeutic

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