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Merck

A3428

Aprotinin

3-8 TIU/mg solid, lyophilized powder, suitable for cell culture

Synonim(y):

BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)

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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C284H432N84O79S7
Numer CAS:
9087-70-1
Masa cząsteczkowa:
6511.44
UNSPSC Code:
12352200
eCl@ss:
32190102
NACRES:
NA.32
EC Number:
232-994-9
MDL number:
MFCD00130541

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Nazwa produktu

Aprotinin from bovine lung, lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell culture

SMILES string

S1SCC2NC(=O)CNC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C5NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C9N(CCC9)C(=O)CNC(=O)C(NC(=O)C(NC(=O)C%11N(CCC%11)C(=O

InChI key

ZPNFWUPYTFPOJU-UHFFFAOYSA-N

biological source

bovine lung

product line

BioReagent

form

lyophilized powder

specific activity

3-8 TIU/mg solid

mol wt

~6,500

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 5 mg/mL

UniProt accession no.

P00974

storage temp.

2-8°C

Quality Level

200

Gene Information

cow ... PTI(404172)

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Application

Aprotinin from bovine lung has been used in insulin and glucagon-like peptide-1 assay. It has also been used a in western blotting.
Aprotinin is largely used as an inhibitor of trypsin.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.
Aprotinin is a natural polypeptide of 58 amino acids. It possesses hemostatic/antiplasmin action and helps to reduce bleeding during surgeries. This prevents the necessity of blood transfusion.

Preparation Note

This product is a lyophilized powder that has been cell culture tested. It has an activity of 3-7 TIU/mg.

Other Notes

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.
When benzoyl-L-arginine ethyl ester (BAEE) is used as substrate one BAPNA unit is approx. 45BAEE μmolar units at pH8.0 at 25°C or approx. 9,000 BAEE A253 units at pH7.6 at 25°C.
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Klasa składowania

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000495849
0000495848
0000495844
0000495847
0000495848

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Produkty

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

Protokoły

Enzymatic Assay of Aprotinin

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

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