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Atto 490LS maleimide
suitable for fluorescence
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
1 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2150,00 zł |
Informacje o tej pozycji
NACRES:
NA.32
UNSPSC Code:
12352111
2150,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócassay
≥90% (HPLC)
Quality Segment
form
(Report result)
mol wt
Mw 818 g/mol
manufacturer/tradename
ATTO-TEC GmbH
transmittance
254 nm, >90%, 490 nm, >90%
fluorescence
λex 496 nm; λem 640.0 nm±10 nm in PBS, pH 7.4
λ
in PBS, pH 7.4
UV absorption
λ: 494-500 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
General description
Atto 490LS is a new fluorescent label featuring an extraordinary large Stokes-Shift of 165 nm. Thus the emission spectrum is almost completely separated from its absorption spectrum, making the dye highly suitable for multiplexing experiments, in particular in combination with Atto 488 and Atto 514. Atto 490LS is very hydrophillic and shows excellent water solubility. The dye exhibits a relatively high fluorescence quantum yield, which is only slightly reduced after conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL). Atto 490LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of -1.
Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labelling of amines, which usually requires a higher pH than reaction of maleimides with thiols.
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Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labelling of amines, which usually requires a higher pH than reaction of maleimides with thiols.
find more information here
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
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Ta pozycja | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| fluorescence λex 496 nm; λem 640.0 nm±10 nm in PBS, pH 7.4 | fluorescence - | fluorescence - | fluorescence - |
| form (Report result) | form solid | form - | form - |
| assay ≥90% (HPLC) | assay ≥90% (HPLC), ≥90% (degree of coupling) | assay ≥90% (HPLC), ≥90% (degree of coupling ) | assay ≥75.0% (HPLC) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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