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Merck

72464

Atto 565 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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1 MG

838,00 zł

838,00 zł


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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C35H34ClN3O11
Masa cząsteczkowa:
708.11
UNSPSC Code:
12352108
NACRES:
NA.32
MDL number:

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product line

BioReagent

assay

≥90% (HPLC), ≥90% (degree of coupling)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

suitability

suitable for fluorescence

detection method

fluorometric

storage temp.

−20°C

Quality Level

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Ta pozycja
796367624567455
suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

-

suitability

suitable for fluorescence

form

powder

form

powder

form

powder

form

solid

assay

≥90% (HPLC), ≥90% (degree of coupling)

assay

≥90.0% (HPLC), ≥90.0% (degree of coupling)

assay

≥90.0% (HPLC)

assay

90% (Coupling efficiency with benzylamine, HPLC)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

-

General description

Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.

Features and Benefits

  • Strong absorption
  • High fluorescence quantum yield
  • High thermal and photo-stability

Other Notes

Fluorescence of bioconjugates[1][2]; employed in fluorescence resonance energy transfer, FRET, experiments[3].

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
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Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Benjamin Zoller et al.
Cell, 175(3), 835-847 (2018-10-20)
How transcriptional bursting relates to gene regulation is a central question that has persisted for more than a decade. Here, we measure nascent transcriptional activity in early Drosophila embryos and characterize the variability in absolute activity levels across expression boundaries.
Michael Cooper et al.
Journal of fluorescence, 14(2), 145-150 (2004-12-24)
The spectral properties of a rigidified trimethine cyanine dye, Cy3B have been characterised. This probe has excellent fluorescent properties, good water solubility and can be bioconjugated. The emission properties of this fluorophore have also been investigated upon conjugation to an
Elizabeth A Jares-Erijman et al.
Nature biotechnology, 21(11), 1387-1395 (2003-11-05)
Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a revised photophysical framework for the phenomenon and provide a systematic catalog of
Rachael Bakker et al.
eLife, 9 (2020-06-23)
Morphogen signaling contributes to the patterned spatiotemporal expression of genes during development. One mode of regulation of signaling-responsive genes is at the level of transcription. Single-cell quantitative studies of transcription have revealed that transcription occurs intermittently, in bursts. Although the
Matthew G Niepielko et al.
Current biology : CB, 28(12), 1872-1881 (2018-06-05)
The formation of ribonucleoprotein assemblies called germ granules is a conserved feature of germline development. In Drosophila, germ granules form at the posterior of the oocyte in a specialized cytoplasm called the germ plasm, which specifies germline fate during embryogenesis. mRNAs

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