62309

Lipase from Pseudomonas cepacia

powder, light beige, ≥30 U/mg

• Synonim(y): PCL, PS Lipase, Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Numer CAS: 9001-62-1
• Numer EC enzymu: 3.1.1.3 (BRENDA, IUBMB)
• Numer WE: 232-619-9
• Numer MDL: MFCD00131509
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: bacterial (Pseudomonas cepacia)
• Poziom jakości: 100
• Postać: powder
• aktywność właściwa: ≥30 U/mg
• warunki przechowywania: (Tightly closed. Dry)
• metody: cell based assay: suitable
• kolor: light beige
• rozpuszczalność: H₂O: 2 mg/mL, hazy, faintly yellow
• numer dostępu UniProt: A0A0J5WKE3
• temp. przechowywania: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• Klucz InChI: QWZUIMCIEOCSJF-CHHCPSLASA-N

Opis

Opis ogólny

Research area: cell-signaling

Lipase is a hydrolytic enzyme, found ubiquitously in nature. It belongs to the α/β-hydrolases fold family. Lipase structure contains amphipathic helical lid domain in the active site that helps in interfacial activation of protein.

Zastosowanie

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Lipase from Pseudomonas cepacian has been used to:

• catalyze the degradation of polycaprolactone scaffold
• catalyze the hydrolysis of Morita-Baylis-Hillman acetates during enzymatic kinetic resolution of racemic Morita-Baylis-Hillman adducts
• as a standard for the generation of a calibration curve to determine the activity of lipase produced by microorganisms isolated from sludge derived from an urban wastewater treatment plant for ethanol production.

Działania biochem./fizjol.

Lipases catalyze the hydrolysis of carboxylic ester bonds in triacylglycerols to yield glycerol and free fatty acids. Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate). Triacylglycerol lipases specifically hydrolyze the outer links of triacylglycerols and operate exclusively on the water-lipid interface. Lipolytic products and intermediates formed during lipolysis are involved in various cell-signaling processes. Lipases have broad substrate specificity and high enantioselectivity. This property of lipase makes it a good catalyst in organic synthesis. Lipases play a vital role in fat digestion and metabolism.

Definicja jednostki

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40 °C (triolein, Cat. No. 62314 as substrate)

Inne uwagi

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Chemoenzymatic synthesis of (-)-carbocyclic 7-deazaoxetanocin G

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)