50981

Guanidine thiocyanate

BioReagent, Molecular Biology, ≥99%

• Synonim(y): Guanidinium rhodanide, Guanidinium thiocyanate
• Wzór liniowy: NH₂C(=NH)NH₂ · HSCN
• Numer CAS: 593-84-0
• Masa cząsteczkowa: 118.16
• UNSPSC Code: 12352107
• NACRES: NA.52
• PubChem Substance ID: 57651291
• EC Number: 209-812-1
• Beilstein/REAXYS Number: 3563461
• MDL number: MFCD00013027

Właściwości

• biological source: synthetic
• Quality Segment: 200
• grade: Molecular Biology
• product line: BioReagent
• assay: ≥99%
• form: powder
• technique(s): RNA extraction: suitable
• impurities: DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected
• color: colorless to white,
• mp: 115-122 °C, 120-122 °C (lit.)
• solubility: H₂O: 4 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤5000 mg/kg
• cation traces: Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤5000 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• storage temp.: room temp
• SMILES string: SC#N.NC(N)=N
• InChI: 1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
• InChI key: ZJYYHGLJYGJLLN-UHFFFAOYSA-N

Opis

Application

Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.

Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) plays a role in protein denaturation.[1] It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP).[2] It can be used along with phenol and chloroform for extraction of RNA.[3]

Guanidine thiocyanate interferes with the hydrogen bonds and intramolecular interactions of macromolecules. It solubilizes the proteins resulting in disintegration of cellular structures.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302 + H312 + H332,H314,H412
• pcodes: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• supp_hazards: EUH032
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves
• Klasa składowania: 8A - Combustible corrosive hazardous materials