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3952016

Sigma-Aldrich

Schiff′s Reagent

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych

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500 ML
507,00 zł

507,00 zł


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500 ML
507,00 zł

About This Item

Kod UNSPSC:
12352106
NACRES:
NA.47

507,00 zł


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Formularz

solution

Poziom jakości

okres trwałości

Expiry date on the label

IVD

for in vitro diagnostic use

pH

<2.0

Zastosowanie

hematology
histology

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Zastosowanie

Intended for use in Sigma Periodic Acid Staining Procedure, 395. Kit is intended for use with blood, bone marrow, tissue touch preparations or routine tissue sections.

Komponenty

Pararosaniline, 1% and sodium metabisulfate, 4%, in hydrochloric acid, 0.25 mol/L

Inne uwagi

Use Sigma reagent S5133 for staining glycoproteins in polyacrylamide gels.
Ta strona może zawierać tekst przetłumaczony maszynowo.

Piktogramy

Health hazardCorrosion

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Carc. 1B - Eye Dam. 1 - Met. Corr. 1

Kod klasy składowania

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Wykazy regulacyjne

Wykazy regulacyjne dotyczą głównie produktów chemicznych. Można w nich podawać ograniczoną liczbę informacji na temat produktów niechemicznych. Brak wpisu oznacza, że żaden ze składników nie znajduje się w wykazie. Użytkownik odpowiada za zagwarantowanie bezpiecznego i zgodnego z prawem stosowania produktu.

EU REACH Annex XVII (Restriction List)


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Naser Mobarra et al.
International journal of hematology-oncology and stem cell research, 8(4), 20-29 (2015-03-17)
The use of stem cells is considered as an appropriate source in cell therapy and tissue engineering. Differentiation of human induced Pluripotent Stem Cells (hiPSCs) to Hepatocyte-like Cells (HLCs) on mouse embryonic fibroblasts (MEFs) feeders is confronted with several problems
Priti Chougule et al.
Scandinavian journal of gastroenterology, 47(11), 1334-1343 (2012-09-05)
Cell culture studies of enterocytes are important in many fields. However, there are difficulties in obtaining cell lines from adult human intestine, such as microbial contamination of cultures from the tissue samples, short life span of enterocytes, overgrowth of mesenchymal
Yael Aylon et al.
Genes & development, 30(7), 786-797 (2016-03-26)
The Hippo signaling pathway is a major regulator of organ size. In the liver, Hippo pathway deregulation promotes hyperplasia and hepatocellular carcinoma primarily through hyperactivation of its downstream effector, YAP. The LATS2 tumor suppressor is a core member of the
Rüdiger Braun et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 26(13), 3468-3480 (2020-04-08)
The standard treatment of patients with locally advanced rectal cancer consists of preoperative chemoradiotherapy (CRT) followed by surgery. However, the response of individual tumors to CRT is extremely diverse, presenting a clinical dilemma. This broad variability in treatment response is
Yan-Yan Song et al.
Oncology letters, 8(1), 431-437 (2014-06-25)
Vasculogenic mimicry (VM) formation is important for invasion and metastasis of tumor cells in gastric adenocarcinoma (GAC). The present study aimed to investigate the association between signal transducer and activator of transcription-3 (STAT3), phosphor-STAT3 (p-STAT3), hypoxia-inducible factor-1α (HIF-1α) and VM

Questions

1–2 of 2 Questions  
  1. Schiff試薬ですが英語の説明書(procedure-395-pas-ug-mk.pdf)では2~8℃で保管となっており、各種染色マニュアルなどとも一致していますが、実際のSchiff試薬瓶(日本で販売されている)には室温保存となっていると思います。これは間違いではないでしょうか?

    1 answer
    1. Product 3952016 was introduced in the late 1970s or early 1980s, solution stability studies were set up. If was determined over time that solutions stored at 2-8°C gave better staining performance.

      Schiff's Reagent stored at room temperature will often not list a color specification for the solution. Product 3952016 will list an appearance specification for color as colorless with a possible faint blue hue. There are other Schiff's reagent available from our website that are stored at room temperature. Product 102572 does not have a color specification for the solution but it does have a specification for SO2. A good Schiff's Reagent should have a smell of rotten eggs due to the presence of SO2 Solutions stored cooler will maintain higher levels of gases as compared to solutions stored at higher temperature. For example: fish kills are common in lakes and rivers when the water temperature becomes to high and available oxygen levels are low. Keeping solutions cooler will results in higher levels of dissolved gases in the Schiff's solution.

      Storage at room temperature is not universal by any means. There are certainly plenty of internet sites that will suggest storage at 2-8°C. But if the Schiff's Reagent is stored at 2-8°C, the solution should be allowed to reach room temperature before staining is performed. It is for ease of use that some companies continue to recommend room temperature storage. There are users that will keep the stock bottle at room temperature but keep a working aliquot at room temperature for daily use. As the solution weakens with time, the solution is discarded and a new aliquot is used for staining. The working solution should never be returned to the stock bottle.

      Helpful?

  2. Does the quality of the product get affected when the Schiff's solution arrives frozen?

    1 answer
    1. In most cases freezing Schiff's solution is not an issue. Freezing Schiff's solution and noticing white precipitate upon thawing requires a complaint and no-charge replacement due to leucopararosaniline crystals, indicating poor staining quality. A solution without white precipitate is suitable. The solution's suitability for use lies in its performance.

      Helpful?

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