Giemsa solution should never be diluted in PBS. Giemsa solution is typically diluted with either deionized water or a low molarity phosphate buffer. Weise and Sorsensen phosphate buffer solutions are typically used. Dilutions can range from 1:10 to 1:80. The most common range for dilutions are either 1:20 or 1:40.
32884
Giemsa Stain
Modified Solution
Synonim(y):
Azure eosin methylene blue, Giemsa solution
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32884-250ML
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Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
MA.02
MDL number:
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Pozwól nam pomócNazwa produktu
Giemsa Stain, Modified Solution, according to Giemsa
form
liquid
Quality Level
quality
according to Giemsa
technique(s)
UV/Vis spectroscopy: suitable
color
dark blue
pH
6.9
density
1.036 g/cm3 at 20 °C
suitability
passes test for UV spectrum
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChI key
NALREUIWICQLPS-UHFFFAOYSA-N
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Ta pozycja | |||
|---|---|---|---|
| suitability passes test for UV spectrum | suitability - | suitability - | suitability - |
| technique(s) UV/Vis spectroscopy: suitable | technique(s) microbe id | staining: suitable | technique(s) - | technique(s) - |
| color dark blue | color - | color - | color - |
| form liquid | form - | form solution | form solution |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) hematology | application(s) hematology |
| Quality Level 200 | Quality Level 200 | Quality Level 500 | Quality Level 500 |
General description
Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.[1]
Application
Modified Giemsa stain may be used for the identification of each human chromosome by differentials staining.[1]
Biochem/physiol Actions
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.
Other Notes
Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.
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signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
target_organs
Eyes,Central nervous system
Klasa składowania
3 - Flammable liquids
wgk
WGK 2
flash_point_f
51.8 °F - closed cup
flash_point_c
11 °C - closed cup
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67-56-1
CAS No.
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To stain a blood smear does this product need to be diluted in PBS? What is the recommended ratio of stain to PBS?
1 answer-
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Hello I would like to ask if this product is suitable for G banding of human chromosomes. Thank you very much!
1 answer-
Product 32884 has not been suitability tested for G banding of human chromosomes. There is no available protocol for use of the product for G banding of human chromosomes. The quality control testing performed on a lot-to-lot basis includes testing for appearance, density, pH, and UV absorbance.
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