18772
Anty-RabbitIgG - Atto 488
whole molecule, 1 mg/mL protein
Synonim(y):
Atto 488-[kozieIgG przeciw królikowi], cała cząsteczka
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Wybierz wielkość
1 ML
2420,00 zł
2420,00 zł
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Informacje o tej pozycji
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
Atto 488 conjugate
Clone:
polyclonal
Application:
ICC, IF, IHC
Citations:
19
Przejdź do
Pomoc techniczna
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Pozwól nam pomócconjugate
Atto 488 conjugate
Quality Level
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
contains
50% glycerol
species reactivity
rabbit
concentration
1 mg/mL protein
technique(s)
immunocytochemistry: 1:500, immunofluorescence: 1:200, immunohistochemistry: suitable
fluorescence
λex 485 nm; λem 552 nm in PBS
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
1 of 4
Ta pozycja | |||
|---|---|---|---|
| conjugate Atto 488 conjugate | conjugate Atto 594 conjugate | conjugate alkaline phosphatase conjugate | conjugate Atto 488 conjugate |
| species reactivity rabbit | species reactivity rabbit | species reactivity rabbit | species reactivity rabbit |
| antibody form affinity isolated antibody | antibody form - | antibody form affinity isolated antibody | antibody form F(ab′)2 fragment of affinity isolated antibody |
| technique(s) immunocytochemistry: 1:500, immunohistochemistry: suitable, immunofluorescence: 1:200 | technique(s) - | technique(s) direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000 | technique(s) - |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in wet ice |
General description
Goat polyclonal anti-Rabbit IgG (whole molecule)-Atto 488 antibody antibody reats with rabbit IgG in vitro, in rabbit serum and biological fluids.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Atto 488 is a labeling dye with high molecular absorption (90,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. It is optimized for excitation with an argon laser, and is characterized by high photostability. Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 488 is a labeling dye with high molecular absorption (90,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. It is optimized for excitation with an argon laser, and is characterized by high photostability. Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Immunogen
Rabbit IgG
Application
Anti-Rabbit IgG has been used for immunocytochemistry applications at a dilution of 1:500 in neural precursor cells.
Goat polyclonal anti-Rabbit IgG (whole molecule)-Atto 488 antibody may be used to detect and quantitate the level of IgG in rabbit serum and biological fluids via fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use rabbit IgG as the primary antibody. Atto 488 goat anti-rabbit IgG antibody was used for immunofluorescence to detect L. monocytogenes in mouse tissue sections following incubation with an anti-listeria antibody. Antibody was used at a 1:200 dilution.
Physical form
Atto 488 goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.
Analysis Note
Unconjugated dye ≤5% of total fluorescence; dye-to-protein ratio >2.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Klasa składowania
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
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Scientific reports, 11(1), 23343-23343 (2021-12-04)
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Javier A Carrero et al.
Infection and immunity, 77(10), 4371-4382 (2009-08-12)
Listeriolysin O (LLO) is an essential virulence factor for the gram-positive bacterium Listeria monocytogenes. Our goal was to determine if altering the topology of LLO would alter the virulence and toxicity of L. monocytogenes in vivo. A recombinant strain was
Janin Lautenschläger et al.
Nature communications, 9(1), 712-712 (2018-02-21)
Alpha-synuclein is known to bind to small unilamellar vesicles (SUVs) via its N terminus, which forms an amphipathic alpha-helix upon membrane interaction. Here we show that calcium binds to the C terminus of alpha-synuclein, therewith increasing its lipid-binding capacity. Using
Jeffrey M Schaub et al.
Langmuir : the ACS journal of surfaces and colloids, 34(49), 14882-14890 (2018-07-26)
Single-stranded DNA (ssDNA) is a critical intermediate in all DNA transactions. Because ssDNA is more flexible than double-stranded (ds) DNA, interactions with ssDNA-binding proteins (SSBs) may significantly compact or elongate the ssDNA molecule. Here, we develop and characterize low-complexity ssDNA
Produkty
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
Powiązane treści
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| 18772-1ML-F | 04061838061874 |
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