T15760

Trizma^® hydrochloride

reagent grade, ≥99%, crystalline

• Synonim(y): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Wzór liniowy: NH₂C(CH₂OH)₃ · HCl
• Numer CAS: 1185-53-1
• Masa cząsteczkowa: 157.60
• Beilstein: 3675235
• Numer MDL: MFCD00012590
• Kod UNSPSC: 12161700
• NACRES: NA.25

Właściwości

• pochodzenie biologiczne: synthetic
• Poziom jakości: 200
• klasa czystości: reagent grade
• Próba: ≥99%
• Postać: crystalline
• warunki przechowywania: (Tightly closed. Dry)
• zanieczyszczenia: <0.5% water
• kolor: white
• przydatny zakres pH: 7.0-9.0
• pKa (25°C): 8.1
• mp: ~150.7 °C
• rozpuszczalność: water: 1.5 g/mL, clear, colorless
• ślady kationów: Fe: ≤5 ppm; Pb: ≤5 ppm
• temp. przechowywania: room temp
• ciąg SMILES: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• Klucz InChI: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Opis

Opis ogólny

Tris HCl, or Trizma^® hydrochloride, serves as a widely utilized acidic buffer with a pKa of 8.08 at 25°C, offering a pH range of 7.0 to 9.0, aligning with the typical pH of living organisms. It is a crucial component in buffer solutions for diverse biological, cell biology, and biochemistry applications. Notably, Tris base and Tris hydrochloride individually lack sufficient buffering capacity, necessitating their combination to create effective buffers within the pH range of 7 to 9.

Essential in protein electrophoresis and western blotting techniques, Tris buffers, particularly low ionic strength versions, are employed for protein transfer. They are also commonly used in SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, integral to DNA agarose electrophoresis, contribute to buffer preparations like TAE (Tris acetate/EDTA) and TBE (Tris borate/EDTA), playing pivotal roles in biochemistry, protein purification, and electrophoresis applications. Overall, Tris HCl proves to be a versatile and indispensable component, ensuring optimal pH in biological systems for effective biochemical and cell biology research.

Cechy i korzyści

• Ideal as a Biological buffer for Cell Biology and Biochemical research
• Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Inne uwagi

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The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

The pH values of all buffers are temperature and concentration-dependent. For Tris buffers, pH increases by about 0.03 unit per °C decrease in temperature and decreases by 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Informacje prawne

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable