PPB015

Tris-Glycine Buffer

pH 8.3, pHast Pack^™, powder

• Synonim(y): 1X TG buffer, TG, TG Buffer, Tris Glycine running buffer, Tris Glycine transfer buffer, Tris glycine
• Kod UNSPSC: 41105319
• NACRES: NA.25

Właściwości

• linia produktu: pHast Pack^™
• Poziom jakości: 100
• Postać: powder
• warunki przechowywania: (Tightly closed. Dry)
• zanieczyszczenia: ≤5 ppm Heavy Metals (lead)
• kolor: white to off-white
• pH: 8.3
• rozpuszczalność: soluble, clear, colorless to faintly yellow
• ślady kationów: Fe: <10 ppm
• obecność zanieczyszczeń: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• temp. przechowywania: room temp

Opis

Opis ogólny

Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.

Zastosowanie

Suitable for use as:

• Transfer buffer component in western blot
• Running buffer in native gel electrophoresis for protein separation
• Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)

Cechy i korzyści

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Saves time and minimizes the effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Opakowanie

Foil pouches

Rekonstytucja

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Przechowywanie i stabilność

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Inne uwagi

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Informacje prawne

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1