C5070

CHAPS hydrate

BioXtra, ≥98% (TLC)

• Synonim(y): 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
• Wzór empiryczny (zapis Hilla): C₃₂H₅₈N₂O₇S · xH₂O
• Numer CAS: 331717-45-4
• Masa cząsteczkowa: 614.88 (anhydrous basis)
• NACRES: NA.21
• PubChem Substance ID: 24892750
• UNSPSC Code: 12161900
• MDL number: MFCD00012116

Właściwości

• description: zwitterionic
• Quality Segment: 400
• product line: BioXtra
• assay: ≥98% (TLC)
• form: powder
• mol wt: 614.88 g/mol (anhydrous basis)
• technique(s): protein purification: suitable, protein quantification: suitable
• impurities: ≤0.001% Phosphorus (P), ≤0.1% Insoluble matter, <0.04% DMF
• ign. residue: ≤0.1%
• mp: 157 °C (dec.) (lit.)
• solubility: H₂O: 0.1 M, clear, colorless
• anion traces: chloride (Cl^-): ≤0.05%
• cation traces: Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH₄⁺: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%
• SMILES string: O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
• InChI key: SJCUTFKCLFLIFE-JWTJKVBLSA-N

Opis

General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

• 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
• 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
• HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable