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PFLRKB

Roche

KAPA PROBE FAST

suitable for qPCR, 2 ×, ROX™ Low

Synonim(y):

Probe qPCR, qPCR

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About This Item

Kod UNSPSC:
41106300
NACRES:
NA.55

Poziom jakości

100

zastosowanie

sufficient for 100 reactions
 sufficient for 1000 reactions
 sufficient for 500 reactions

okres trwałości

≤12 mo.

Właściwości

dNTPs included
hotstart

opakowanie

kit of 1 mL (100 x 20 μL rxn; KK4716)
kit of 10 mL (1000 x 20 μL rxn; KK4718)
kit of 5 mL (500 x 20 μL rxn; KK4717)
pkg of

producent / nazwa handlowa

Roche

stężenie

2 ×

metody

qPCR: suitable

moc wejściowa

purified DNA

kompatybilność

for use with Agilent Mx3000P
for use with Agilent Mx3005P
for use with Agilent Mx4000
for use with Applied Biosystems® 7500
for use with Applied Biosystems® ViiA 7
for use with QuantStudio™

metoda wykrywania

probe-based

temp. przechowywania

−20°C

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Opis ogólny

KAPA PROBE FAST qPCR Kits provide fast and reproducible results for all probe-based qPCR applications. Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g. TaqMan®), FRET probes, and displacement probes (e.g. molecular beacons).

Zastosowanie

KAPA PROBE FAST qPCR Master Mix (2X) with ROX™ Low is suitable for use in:
  • Genotyping
  • Gene expression analysis
  • Multiplex qPCR
  • SNP genotyping/allelic discrimination
  • Microarray validation
  • Real time(RT)-quantitative polymerase chain reaction (qPCR)/qPCR

Cechy i korzyści

Accurately discriminate between alleles:
  • Exceptional differentiation of heterozygous and homozygous alleles
  • Discrete clusters in SNP genotyping assays

Achieve excellent reproducibility and efficiency:
  • Fast, high performance five-colored multiplex PCR
  • Similar abundance levels achieved with low copy number
  • Broad dynamic range of up to 10 orders of magnitude

Increase flexibility:
  • Compatible with both standard and fast cycling protocolsRetain accurate quantification

Quick Notes:
  • These kits are compatible with all fluorogenic probebased technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan®) and displacement probes (e.g., molecular beacons).
  • Initial denaturation for 20 sec at 95ºC is sufficient for enzyme reactivation; however, optimal denaturation of complex targets may require up to 3 min denaturation.
  • For 2-step cycling, use 30 sec combined annealing/ extension/data acquisition.
  • For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition.
  • Do not exceed 25 μL volumes.

Jakość

KAPA PROBE FAST qPCR Master Mix (2X) is subjected to stringent quality control tests, is free of contaminating exoand endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Uwaga dotycząca przygotowania

ROX/fluorescein reference dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity. Avoid repeated freezing and thawing. Always ensure that the product has been fully thawed and mixed before use.

KAPA PROBE FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week, and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.

Inne uwagi

For Research Use Only. Not for use in diagnostic procedures.

Informacje prawne

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
QuantStudio is a trademark of IROA Technologies LLC
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
This page may contain text that has been machine translated.

Tylko elementy zestawu

Numer produktu
Opis
  • KAPA PROBE FAST qPCR Master Mix (2X) with ROX™ Low
  • KAPA Taq HotStart® DNA Polymerase
  • reaction buffer
  • dNTPs
  • MgCl2 5 nM

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

does not flash

Temperatura zapłonu (°C)

does not flash

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Odwiedź Bibliotekę dokumentów

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T helper type 2 (Th2) cells are important regulators of mammalian adaptive immunity and have relevance for infection, autoimmunity, and tumor immunology. Using a newly developed, genome-wide retroviral CRISPR knockout (KO) library, combined with RNA-seq, ATAC-seq, and ChIP-seq, we have dissected
Sydney Lavoie et al.
Gastroenterology, 158(5), 1359-1372 (2020-01-10)
Intestinal microbes and their metabolites affect the development of colorectal cancer (CRC). Short-chain fatty acids are metabolites generated by intestinal microbes from dietary fiber. We investigated the mechanisms by which free fatty acid receptor 2 (FFAR2), a receptor for short-chain

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