FSSGMMRO
Roche
FastStart SYBR Green Master
sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR
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About This Item
Kod UNSPSC:
41106300
NACRES:
NA.55
Polecane produkty
zastosowanie
sufficient for 500 reactions
sufficient for 5000 reactions
Poziom jakości
Właściwości
dNTPs included: no
hotstart
producent / nazwa handlowa
Roche
opakowanie
pkg of 500 x 20 μL reactions (04673484001)
pkg of 5000 x 20 μL reactions (04673492001)
metody
RT-qPCR: suitable
qPCR: suitable
moc wejściowa
purified DNA
metoda wykrywania
probe-based
Powiązane kategorie
Opis ogólny
FastStart SYBR Green Master; Instructions For Use
FastStart™ SYBR® Green Master is a ready-to-use hot start reaction mix without ROX for quantitative polymerase chain reaction (qPCR) and reverse transcription (RT)-qPCR on real-time PCR systems other than the LightCycler® instruments. This master mix simplifies the preparation of reactions for DNA detection and analysis. In combination with a real-time PCR instrument, suitable PCR primers, and a hydrolysis probe, FastStart™ TaqMan® Probe Master allows very sensitive detection and quantification of defined DNA sequences.
SYBR® Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis, the SYBR® Green I dye, included in the reaction mix, binds to the amplified PCR products. The amplicon can be detected by its fluorescence.
Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart™ Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can non-specifically bind. The FastStart™ Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).
The FastStart™ SYBR® Green Master can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich. However, you would need to adapt your detection protocol to the reaction conditions of the particular real-time PCR instrument used and design specific PCR primers for each target.
Combine this master mix with our Transcriptor First Strand cDNA Synthesis Kit to achieve excellent results in two-step qRT-PCR.
FastStart™ SYBR® Green Master is a ready-to-use hot start reaction mix without ROX for quantitative polymerase chain reaction (qPCR) and reverse transcription (RT)-qPCR on real-time PCR systems other than the LightCycler® instruments. This master mix simplifies the preparation of reactions for DNA detection and analysis. In combination with a real-time PCR instrument, suitable PCR primers, and a hydrolysis probe, FastStart™ TaqMan® Probe Master allows very sensitive detection and quantification of defined DNA sequences.
SYBR® Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis, the SYBR® Green I dye, included in the reaction mix, binds to the amplified PCR products. The amplicon can be detected by its fluorescence.
Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart™ Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can non-specifically bind. The FastStart™ Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).
The FastStart™ SYBR® Green Master can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich. However, you would need to adapt your detection protocol to the reaction conditions of the particular real-time PCR instrument used and design specific PCR primers for each target.
Combine this master mix with our Transcriptor First Strand cDNA Synthesis Kit to achieve excellent results in two-step qRT-PCR.
Zastosowanie
The FastStart™ SYBR® Green Master has been used in qPCR and two-step qRT-PC in the SYBR® Green I detection format. It is also used:
Use the FastStart™ SYBR® Green Master with any real-time qPCR instrument other than the LightCycler® Instruments.
- in quantitative polymerase chain reaction (qPCR) for adeno-associated virus (AAV) titre quantification
- in qPCR to determine the expression level of different Col6a1-3 genes relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH)
- in quantitative real-time polymerase chain reaction (qRT-PCR) of G protein-coupled estrogen receptor-1 (GPER) mRNA
- in qRT-PCR for gene expression studies
Use the FastStart™ SYBR® Green Master with any real-time qPCR instrument other than the LightCycler® Instruments.
Cechy i korzyści
- Improve PCR sensitivity and specificity:
- Avoid over-estimation of qPCR results:
- Amplify and detect a broad range of DNA or cDNA targets:
- Use any real-time PCR instrument other than the LightCycler® Instruments:
- Save time and effort in qPCR preparation:
- Prevent false positives resulting from carryover contamination:
Komponenty
FastStart SYBR Green Master, 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), and SYBR Green I.
Jakość
Function test: Each lot is tested for performance in qPCR using three templates: a GC-rich template, an AT-rich template, and a long template (about 440 bp).
Inne uwagi
qPCR targets
In principle, the FastStart SYBR Green Master can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich. However, for each target, you would need:
See the Operator′s Manual of your real-time PCR instrument for general recommendations.
Two forms available
The master mix is available in two forms – one that contains the ROX reference dye and one without ROX.
Preventing carryover contamination
This master contains dUTP, which will be incorporated into PCR products to help prevent false positives resulting from carryover contamination. In subsequent PCRs, you can add Uracil-DNA Glycosylase to degrade any uracil-containing carryover contaminants (amplification products from previous PCRs).
qRT-PCR
Combine this master mix with our Transcriptor First Strand cDNA Synthesis Kit for two-step qRT-PCR. This kit gives excellent results and works efficiently with all real-time PCR instruments.
In principle, the FastStart SYBR Green Master can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich. However, for each target, you would need:
- to adapt your detection protocol to the reaction conditions of your real-time PCR instrument, and
- design specific PCR primers for the target.
See the Operator′s Manual of your real-time PCR instrument for general recommendations.
Two forms available
The master mix is available in two forms – one that contains the ROX reference dye and one without ROX.
Preventing carryover contamination
This master contains dUTP, which will be incorporated into PCR products to help prevent false positives resulting from carryover contamination. In subsequent PCRs, you can add Uracil-DNA Glycosylase to degrade any uracil-containing carryover contaminants (amplification products from previous PCRs).
qRT-PCR
Combine this master mix with our Transcriptor First Strand cDNA Synthesis Kit for two-step qRT-PCR. This kit gives excellent results and works efficiently with all real-time PCR instruments.
For life science research only. Not for use in diagnostic procedures.
Informacje prawne
FastStart is a trademark of Roche
LightCycler is a registered trademark of Roche
SYBR is a registered trademark of Life Technologies
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
This page may contain text that has been machine translated.
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Produkty
Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.
Obejrzyj te filmy, aby dowiedzieć się, jak działa PCR w czasie rzeczywistym lub ilościowy PCR (qPCR) i jakie są zalety zarówno metod opartych na SYBR Green, jak i metod opartych na sondach qPCR.
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
Mieszanina wzorcowa PCR upraszcza PCR/RT-PCR dzięki składnikom takim jak polimeraza DNA, dNTP, MgCl2 i bufor, dostępnym w handlu lub DIY.
Powiązane treści
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
Reakcja łańcuchowa polimerazy (PCR) jest techniką amplifikacji cząsteczek kwasów nukleinowych i jest powszechnie stosowana w wielu aplikacjach, w tym RT-PCR, hot start PCR, end point PCR i innych.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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