The concentration provided at 200uM in DMSO solution, final volume 50ul. Please spin down this material before usage to be able to collect all the solution in the bottom of the tube. It is recommended to use the material at 1:1000 dilution, but this is sample dependent.
SCT083
BioTracker™ MemBright 488 Live Cell Dye
Synonim(y):
Live cell imaging probe, Live cell membrane probe, green
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
1 vial | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 902,00 zł |
Informacje o tej pozycji
NACRES:
NA.47
UNSPSC Code:
41106514
902,00 zł
Dostępne do wysyłki DZISIAJSzczegóły
Pomoc techniczna
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Pozwól nam pomócmol wt
observed mol wt 1218.5 kDa
Quality Segment
packaging
vial of 50 μL
manufacturer/tradename
Millipore
growth mode
N/A
technique(s)
cell based assay: suitable, flow cytometry: suitable
detection method
fluorometric
shipped in
dry ice
storage temp.
−20°C
General description
The BioTracker™ Membright-488 live cell dye is a “turn-on” fluorescence probe emitting in the far red spectrum for the imaging of plasma membranes of live cells.
Specific plasma membrane markers are essential for delineating the cell. Membright dyes exploit a unique fluorogenic mechanism, existing in a self-quenched nanoparticle state. Fluorescence is turned on upon contact with and propagation within the plasma membrane, producing bright, background-free signal. The Membright live cell membrane dyes are compatible with two-photon imaging, superresolution imaging, and fixed cell preparations. The high photostability and lack of cytotoxicity of Membright dyes make them suitable for long-term live cell imaging. The suite of superior characteristics of Membright plasma membrane dyes permit use in a wide spectrum of fluorescence microscopy applications and flow cytometry.
Spectral Properties
Fluorescence images obtained by λex = 488 nm and emission range of 500-550 nm.
Reference
Collot M et al. MemBright: A family of fluorescent membrane probes for advanced cellular imaging and neuroscience. Cell Chem. Biol. 2019, 26:600-614
Specific plasma membrane markers are essential for delineating the cell. Membright dyes exploit a unique fluorogenic mechanism, existing in a self-quenched nanoparticle state. Fluorescence is turned on upon contact with and propagation within the plasma membrane, producing bright, background-free signal. The Membright live cell membrane dyes are compatible with two-photon imaging, superresolution imaging, and fixed cell preparations. The high photostability and lack of cytotoxicity of Membright dyes make them suitable for long-term live cell imaging. The suite of superior characteristics of Membright plasma membrane dyes permit use in a wide spectrum of fluorescence microscopy applications and flow cytometry.
Spectral Properties
Fluorescence images obtained by λex = 488 nm and emission range of 500-550 nm.
Reference
Collot M et al. MemBright: A family of fluorescent membrane probes for advanced cellular imaging and neuroscience. Cell Chem. Biol. 2019, 26:600-614
Application
Purity: > 98% confirmed by HPLC, HNMR, LC-MS and elemental analysis.
Features and Benefits
Green fluorescent probe for efficient, selective labeling of the plasma membrane in live cells. Suitable for superresolution microscopy.
Physical form
Green solution
Preparation Note
Store long term at -20°C, protected from light.
Other Notes
Live cell fluorescent imaging, flow cytometry
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja | |||
|---|---|---|---|
| description - | description - | description - | description - |
| technique(s) cell based assay: suitable, flow cytometry: suitable | technique(s) cell based assay: suitable, flow cytometry: suitable | technique(s) cell based assay: suitable, flow cytometry: suitable | technique(s) cell based assay: suitable, cell culture | stem cell: suitable, flow cytometry: suitable |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C (−15°C to −25°C) |
| shipped in dry ice | shipped in dry ice | shipped in cooler | shipped in dry ice |
| growth mode N/A | growth mode N/A | growth mode N/A | growth mode N/A |
| manufacturer/tradename Millipore | manufacturer/tradename Millipore | manufacturer/tradename Millipore | manufacturer/tradename Millipore |
Klasa składowania
10 - Combustible liquids
wgk
WGK 1
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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What is the concentration of membright 488. Only info I have is that it is 50 microlitres. Publications use it at 20 nM
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