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SCR012

PluriStem 129/S6 Murine ES cells

The PluriStem 129S6 Murine ES cell line is derived from the widely used 129/S6/SvEv strain of mice (at 3.5 days p.c.) & is intended for injection into black or albino host blastocysts.

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Informacje o tej pozycji

UNSPSC Code:
41106509
NACRES:
NA.81
eCl@ss:
32011203
Biological source:
mouse
Pomoc techniczna
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Pozwól nam pomóc

biological source

mouse

Quality Level

100

manufacturer/tradename

Chemicon®, PluriSTEM®

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)

shipped in

liquid nitrogen

storage temp.

-140 to -196°C

General description

The PluriStem 129S6 Murine ES cell line is derived from the widely used 129/S6/SvEv strain of mice (at 3.5 days p.c.) and is intended for injection into black or albino host blastocysts. This cell line is characterized by the production of highly chimeric, germline-competent mice. Cells are supplied at passage 10.

Complete disease testing and karyotype data is available under Information Library tool bar, Tech Notes at SCR012 product specific page at http://www.chemicon.com/Product/ProductDataSheet.asp?ProductItem=SCR012.

STRAIN: 129/S6/SvEv

GENDER: Male

KARYOTYPE: 40XY

Application

Research Category
Stem Cell Research

Biochem/physiol Actions

Embryonic Stem Cells

Packaging

2.5 x 106 cells per vial

Physical form

Cells are supplied frozen in basal medium supplemented with 10% FCS and 10% DMSO

Preparation Note

Place vials in liquid Nitrogen immediately upon receipt until it is convenient to proceed to subculture.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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Protokoły

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Powiązane treści

Murine Embryonic Stem Cell Culture Procedures & Protocols

The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.

Improved Productivity of Mouse Embryonic Stem Cell Culture Using Millicell HY Multilayer Culture Flasks

Traditionally, culture and expansion of mouse embryonic stem cells (mESC) is performed using several single-layer T-flasks or Petri dishes. Harvesting and analyzing stem cells from multiple culture vessels is a repetitive and time-intensive process that consumes valuable incubator space. Here, we report a method for expanding mESCs using a new multilayer cell culture flask. The Millicell HY multilayer culture flask makes expansion and harvesting of mESCs faster and easier than traditional culture methods. The method for using the new flasks is linearly scalable per unit surface area. Therefore, no reoptimization of procedure is required – culture conditions and media volume are identical, regardless of whether culture is performed in traditional single-layer devices or in a Millicell HY culture flask. Resulting morphology, cell recovery, viability, and pluripotency of mESCs were not significantly different between multilayer flask culture and single-layer flask culture methods.

The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling

"As transgenic mouse core laboratories know well, mouse models are a powerful means of determining the function of mammalian genes in the context of whole organisms—especially since over 95% of the mouse genome is similar to the human genome. However, you may not know that: 1. Laboratory mouse strains are descended from ancestors in modern-day Pakistan. 2. The first inbred strain of lab mouse was created in 1909. It was named DBA for its coat color: dilute brown non-agouti. 3. There is a mutant mouse actually named“Bad Hair Day” (Bhrd). Someone get this little guy a stylist! 4. Male mice, when treated with anticancer drugs, have been shown to pass on DNA instability to their offspring. 5. The founder of Jackson Laboratories, C. C. Little, once had lunch with Walt Disney to request that an animated film be created linking Mickey Mouse and the laboratory mouse. The request was denied."

Ting Li et al.
Oncotarget, 6(31), 31805-31819 (2015-09-18)
Activation of IκB kinase β (IKK-β) and nuclear factor (NF)-κB signaling contributes to cancer pathogenesis and inflammatory disease; therefore, the IKK-β-NF-κB signaling pathway is a potential therapeutic target. Current drug design strategies focus on blocking NF-κB signaling by binding to
Tao Deng et al.
Development, growth & differentiation, 49(7), 603-610 (2007-07-20)
The completely embryonic stem (ES) cell-derived mice (ES mice) produced by tetraploid embryo complementation provide us with a rapid and powerful approach for functional genome analysis. However, inbred ES cell lines often fail to generate ES mice. The genome of
Babita Madan et al.
Molecular and cellular biology, 29(11), 3186-3203 (2009-04-01)
Dppa4 (developmental pluripotency-associated 4) has been identified in several high-profile screens as a gene that is expressed exclusively in pluripotent cells. It encodes a nuclear protein with an SAP-like domain and appears to be associated preferentially with transcriptionally active chromatin.
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Numer pozycji handlu globalnego

SKUNUMER GTIN
SCR01204053252465482