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S7113

Sigma-Aldrich

DAPI/Antifade Solution, Ready to Use

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About This Item

Kod UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.47

producent / nazwa handlowa

Chemicon®

Poziom jakości

metoda wykrywania

fluorometric

Warunki transportu

dry ice

Opis ogólny

DAPI: 4′, 6-Diamino-2-phenylindole dihydrochloride. Antifade Solution: p-Phenylenediamine and glycerol in PBS solution. 0.4μg/mL DAPI suspended in Antifade. The product is ready to add to fixed slides or cells cultures.

Zastosowanie

A nuclear fluorescent stain used for counterstaining paraffin-embedded, formalin-fixed tissue, metaphase chromosomes, or interphase nuclei that have been hybridized with probes detected with Fluorescein, or equivalent stains. Antifade prevents the permanent loss of fluorescence or fluorescent signal quenching due to photobleaching and prolonged exposure to high intensity light sources.

DAPI binds the minor groove of DNA at AT clusters and binds preferentially to ds DNA (Biochemistry 26:4545, 1987).

Materials Provided

Each vial contains 1 ml of DAPI/Antifade Solution

Warnings and Precautions

DAPI is a potential carcinogen. Antifade is a potential toxin and irritant. Use extreme caution. Avoid contact with eyes, skin and mucous membranes. Use extreme caution. Wear gloves and a lab coat when handling. Follow your institution′s guidelines for hazardous waste disposal. Keep tightly capped and limit exposure to light.



Related Products

Antifade Solution S7114

Propidium Iodide Solution S7109

Propidium Iodide/Antifade Solution S7112

Postać fizyczna

Liquid PBS pH 8.0 containing ~50% glycerol and 0.4μg/mL of DAPI and 2-7mM phenylenediamine.

Przechowywanie i stabilność

Store at -20ºC in undiluted aliquots for up to 1 year from date of receipt. Protect from light

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Aquatic Chronic 3

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2


Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

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Bio-medical materials and engineering null
Creation of Biofunctionalized Micropatterns on Poly(methyl methacrylate) by Single-Step Phase Separation Method.
Ho QP, Wang SL, Wang MJ
ACS Applied Materials & Interfaces null
Yusuke Kambe et al.
Biomaterials, 31(29), 7503-7511 (2010-07-21)
Initial chondrocyte-silk fibroin interactions are implicated in chondrogenesis when using fibroin as a scaffold for chondrocytes. Here, we focused on integrin-mediated cell-scaffold adhesion and prepared cell adhesive fibroin in which a tandem repeat of the Arg-Gly-Asp-Ser (RGDS) sequence was genetically
Mohammadhossein Khorraminejad-Shirazi et al.
Stem cell research & therapy, 11(1), 45-45 (2020-02-06)
Mesenchymal stromal cell (MSC) stemness capacity diminishes over prolonged in vitro culture, which negatively affects their application in regenerative medicine. To slow down the senescence of MSCs, here, we have evaluated the in vitro effects of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), an
Kuan-Jung Chen et al.
Journal of cell science, 133(3) (2020-01-15)
Cell migration requires the coordination of multiple signaling pathways involved in membrane dynamics and cytoskeletal rearrangement. The Arf-like small GTPase Arl4A has been shown to modulate actin cytoskeleton remodeling. However, evidence of the function of Arl4A in cell migration is

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