MABT496
Anti-PKN2, clone 1D1 Antibody
ascites fluid, clone 1D1, from mouse
Synonim(y):
Serine/threonine-protein kinase N2, Protein kinase C-like 2, Protein-kinase C-related kinase 2, PKN2, PRK2, PRKCL2
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
1D1, monoclonal
reaktywność gatunkowa
mouse, rat, monkey, human
metody
ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... PKN2(5586)
Powiązane kategorie
Opis ogólny
Serine/threonine-protein kinase N2, also known as Protein kinase C-like 2 or Protein-kinase C-related kinase 2, and encoded by the gene name PKN2/PRK2/PRKCL2, is a PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. PKN2 has been shown to play an important role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Protein-kinase-C-related kinases (PRKs) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN. Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis. PKN2 is expressed in numerous tumor cell lines, especially in bladder tumor cells.
Immunogen
Purified recombinant fragment of human PKN2 expressed in E. Coli
Zastosowanie
Anti-PKN2, clone 1D1 Antibody is an antibody against PKN2 for use in western blotting, ELISA, IHC, flow cytometry.
ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Immunohistochemistry Analysis: A 1;200-1,000 dilution from a representative lot detected PKN2 in prostate and lung cancer tissues.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected PKN2 in NIH/3T3 cells.
Immunohistochemistry Analysis: A 1;200-1,000 dilution from a representative lot detected PKN2 in prostate and lung cancer tissues.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected PKN2 in NIH/3T3 cells.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Jakość
Evaluated by Western Blotting in PC-12, Cos7, K562, Jurkat, HeLa, A431, C6, NIH/3T3 and HEK293 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PKN2 in PC-12, Cos7, K562, Jurkat, HeLa, A431, C6, NIH/3T3 and HEK293 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PKN2 in PC-12, Cos7, K562, Jurkat, HeLa, A431, C6, NIH/3T3 and HEK293 cell lysates.
Opis wartości docelowych
~140 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Postać fizyczna
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Hitesh Patel et al.
Scientific reports, 10(1), 3902-3902 (2020-03-05)
PRK1 and PRK2 are two closely related AGC-family serine/threonine protein kinases. Here we demonstrate novel roles for them at cilia and in cancer biology. In both instances serum withdrawal leads to increased activating PRK1 and PRK2 phosphorylation (pPRK1/pPRK2) and their
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