MABT20

Anti-OL-protocadherin Antibody, clone 5G10

clone 5G10, from rat

• Synonim(y): protocadherin 10, ortholog of OL-pcdh
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 5G10, monoclonal
• Application: ICC, IHC, WB
• Citations: 9

Właściwości

• biological source: rat
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5G10, monoclonal
• species reactivity: mouse
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001091641
• UniProt accession no.: Q925I8
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Pcdh10(18526)

Opis

General description

OL-protocadherin (OL-pc, protocadherin 10) is a transmembrane protein and a member of the cadherin superfamily, which is characterized by its members’ abilities to undergo interactions that are homophilic in nature via their extracellular domain. Several protocadherins have been observed within the nervous system, and a few have been shown to localize at synapses. With OL-pc, the homophilic binding has been shown to stimulate cell aggregation when the protein is introduced into cadherin-deficient cells. Although the function of OL-protocadherin has yet to be elucidated, studies have shown OL-pc to be necessary to axonal normal projection through the ventral telencephalon, where OL-pc shows highest expression at the striatal region.

~ 130 kDa observed

Immunogen

Epitope: Cytoplasmic domain

GST-tagged recombinant protein corresponding to the cytoplasmic domain of mouse OL-protocadherin.

Application

Anti-OL-protocadherin Antibody, clone 5G10 is an antibody against OL-protocadherin for use in WB, IC, IH.

Immunofluorescence Analysis: The antibody has been shown to detect OL-protocadherin in IF. (Nakao, 2008).

Immunohistochemistry Analysis: The antibody has been shown to detect OL-protocadherin in IH. (Aoki, 2003).

Research Category
Cell Structure

Neuroscience

Research Sub Category
Adhesion (CAMs)

Synapse & Synaptic Biology

Biochem/physiol Actions

The antibody recognizes the cytoplasmic domain of OL-protocadherin.

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse E13-E14 brain tissue lysate

Evaluated by Western Blot in mouse E13-E14 brain tissue lysate.

Western Blot Analysis: 1 µg/mL of the antibody detected OL-protocadherin in 10 µg of mouse E13-E14 brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable