MABS1697

Anti-WISP-3 Antibody, clone 13C4.1

clone 13C4.1, from mouse

• Synonim(y): WNT1-inducible-signaling pathway protein 3, CCN family member 6, WISP-3
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 13C4.1, monoclonal
• reaktywność gatunkowa: mouse, human, rat
• metody: immunohistochemistry: suitable (paraffin); western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_003871
• numer dostępu UniProt: O95389
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... CCN6(8838)

Opis

Opis ogólny

WNT1-inducible-signaling pathway protein 3 (UniProt O95389; also known as CCN family member 6, WISP-3) is encoded by the WISP3 (also known as CCN6, LIBC, PPAC, PPRD, UNQ462/PRO790/PRO956) gene (Gene ID 8838) in human. WISP-3 is a member of the CCN (CTGF, Cyr61, NOV) family connective tissue growth modulators involved in cell growth/differentiation during various phases of development through a complex interplay with BMP/Notch/Wnt signaling pathways. WISP-3 is expressed in cells and tissues of mesenchymal origin and WISP3 gene mutations are linked to an autosomal recessive skeletal disorder known as progressive pseudorheumatoid dysplasia (PPRD), indicating its importance in skeletal growth and the maintenance of cartilage integrity. WISP-3 interacts with BMP and components of the Wnt1 signaling pathway including LRP6 and Fz8, promoting the expression of cartilage-specific proteins collagen-II and aggrecan. Both IGF1 and ROS stimulate WISP-3 expression, WISP-3 in turn binds to and blocks IGF1 secretion, preventing IGF1-induced collagen X induction, ROS accumulation, and alkaline phosphatase activity, all of which are associated with chondrocyte hypertrophy induction. Human WISP-3 is synthesized with a signal peptide sequence (a.a. 1-23), the removal of which yields the mature protein containing several protein-interacting domains, including an IGF-interacting IGFBP domain (a.a. 44-117) with a von Willbrand factor type C repeat, a Thrombospondin (TSP) type-1 domain (a.a. 208-253), and a C-terminal cystine knot-like (CTCK) domain (a.a. 268-342).

Specyficzność

Clone 13C4.1 targets an epitope within the WISP-3 IGFBP domain.

Immunogen

GST-tagged recombinant human WISP-3 IGFBP domain fragment.

Zastosowanie

Detect WISP-3 using this mouse monoclonal Anti-WISP-3, clone 13C4.1, Cat. No. MABS1697, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50-1,000 dilution of this antibody detected WISP-3 in human liver, kidney, and lung tissue sections.

Jakość

Evaluated by Western Blotting in rat kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WISP-3 in 10 µg of rat kidney tissue lysate.

Opis wartości docelowych

~39 kDa observed. 36.84/36.33 kDa (human/rat mature form), 39.29/41.40 kDa (human isoform 1/2 pro-form), and 38.92 kDa (rat pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Inne uwagi

Concentration: Please refer to lot specific datasheet.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1