MABS1283

Anti-MLN64/STARD3 Antibody, clone 2BE-2F4

ascites fluid, clone 2BE-2F4, from mouse

• Synonim(y): StAR-related lipid transfer protein 3, MLN64/STARD3, Metastatic lymph node gene 64 protein, MLN 64, Protein CAB1, START domain-containing protein 3, StARD3
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: ascites fluid
• rodzaj przeciwciała: primary antibodies
• klon: 2BE-2F4, monoclonal
• reaktywność gatunkowa: human
• metody: electron microscopy: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_001159409
• numer dostępu UniProt: Q14849
• Warunki transportu: dry ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... STARD3(10948)

Opis

Opis ogólny

MLN64/StARD3 (also known as es64, StAR-related lipid transfer protein 3, Metastatic lymph node gene 64 protein, Metastatic lymph node protein 64, StARD3, START domain-containing protein 3, Steroidogenic acute regulatory protein related; UniProt Q14849) is encoded by the MLN64 (or CAB1 & STARD3) gene in human (NCBI Gene ID 10948). MLN64/StARD3 is a late endosome (LE) multitransmembrane protein that plays an important role in intracellular cholesterol transport and steroidogenesis. In addition to binding cholesterol via its C-terminal STAR domain, MLN64/StARD3 is reported to interact with ER-anchored VAP proteins via a conserved motif called "two phenylalanines in an acidic tract" or FFAT, thereby forming an LE-ER tethering complex at the inter-organelle membrane contacts sites (MCSs), allowing heterologous membrane apposition and the exchange of metabolites (PMID 23709693 and 24105263). Clone 2BE-2F4 is also known as 2BE2F4 and mAbMLN64-Ct, it detects an epitope within the c-terminal region encompassing the last 20% of MLN64 sequence.

Immunogen

Epitope: Near C-terminus

Ovalbumin-conjugated linear peptide corresponding to human MLN64/STARD3 near the C-terminus.

Zastosowanie

Anti-MLN64/STARD3 Antibody, clone 2BE-2F4 is an antibody against MLN64/STARD3 for use in Western Blotting and Immunocytochemistry, Immunohistochemistry, Electron Microscopy.

Immunocytochemistry Analysis: A representative lot detected MLN64/STARD3 in MCF7/MLN64 cells (Alpy, F., et al. (2001). J. Biol. Chem. 276:4261-4269).
Immunocytochemistry Analysis: A representative lot detected MLN64/STARD3 in BT-474, MCF7, and transfected COS-1 cells (Lutz-Moog, C., et al. (1997). Int. J. Cancer. 71:183-191).
Immunohistochemistry Analysis: A representative lot detected MLN64/STARD3 in human breast tissue (Lutz-Moog, C., et al. (1997). Int. J. Cancer. 71:183-191).
Electron Microscopy Analysis: A representative lot detected MLN64/STARD3 in HeLa cells (Holtta-Vuori, M., et al. (2005). Molecular Biology of the Cell. 16:3873-3886).
Western Blotting Analysis: A representative lot detected MLN64/STARD3 in COS-1 cell lysate (Watari, H., et al. (1997). Proc. Natl. Acad. Sci. USA. 94:8462-8467).
Western Blotting Analysis: A representative lot detected MLN64/STARD3 in various human cell lines (Lutz-Moog, C., et al. (1997). Int. J. Cancer. 71:183-191).

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Jakość

Evaluated by Western Blotting in SK-OV-3 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected MLN64/STARD3 in 10 µg of SK-OV-3 cell lysate.

Opis wartości docelowych

~50 kDa observed. This protein has isoforms at ~48 and ~49 kDa, which are covered by this antigen.

Postać fizyczna

Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.

Unpurified

Przechowywanie i stabilność

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable