MABN750
Anti-VANGL2 Antibody, clone 2G4
clone 2G4, from rat
Synonim(y):
Vang-like protein 2, Loop-tail-associated protein, Loop-tail protein 1, Strabismus 1, Van Gogh-like protein 2
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rat
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
2G4, monoclonal
reaktywność gatunkowa
human, mouse
reaktywność gatunkowa (przewidywana na podstawie homologii)
rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
metody
ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG2aκ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
ambient
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... VANGL2(57216)
mouse ... Vangl2(93840)
Opis ogólny
Vang-like protein 2 (UniProt Q91ZD4; also known as Loop-tail-associated protein, Loop-tail protein 1, Van Gogh-like protein 2) is encoded by the Vangl2 (also known as Lpp1, Ltap, Stb1, Stbm) gene (Gene ID 93840) in murine species. Mammalian Van Gogh-like proteins (VANGL1 & VANGL2) are orthologues of Vangl/Strabismus originally identified as a core planar-cell-polarity (PCP) gene in Drosophila. VANGL1 & VANGL2 are proteins of the WNT/PCP pathway that acts as a key regulator of cell polarity and directional movements that plays a key role in tissue morphogenesis and embryonic development. PCP is involved in neural tube closure, in the orientation of hair bundle in inner ear sensory cells and of motile cilia in the embryonic node, as well as in asymmetric cell division. VANGL1 and VANGL2 missense mutations have been identified in human embryos affected with severe neural tube defects (NTD). Likewise, homozygous Vangl2 Looptail mutation (Lp) in mice causes morphogenesis and patterning defects in numerous tissues, including the most severe failure in neural tube closure (craniorachischisis). VANGL1 and VANGL2 are both 4-transmembrane (a.a. 109-129, 148-168, 179-199, and 218-238 of murine VANGL2) plasma membrane proteins with cytoplasmic N- and C-terminal ends (a.a. 1-108 and 239-521 of murine VANGL2), they show overlapping, but not identical expression patterns in mouse brain. Both proteins can homo- or heterodimerize, creating different complexes during embryonic development.
Specyficzność
Clone 2G4 reacted specifically with VANGL2, but not VANGL1. Clone 2G4 detected shRNA-mediated VANGL2 downregulation in SK-BR-7 human breast carcinoma cells (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
Immunogen
GST-tagged recombinant VANGL2 N-terminal fragment 100% conserved among human, mouse, and rat species.
Zastosowanie
Anti-VANGL2 Antibody, clone 2G4, Cat. No. MABN750, is a highly specific rat monoclonal antibody that targets VANGL2 and has been tested in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blotting.
ELISA Analysis: Clone 2G4 hybridoma culture supernatant specifically detected VANGL2, but not VANGL1, N-terminal fragment GST fusion (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
Immunofluorescence Analysis: A representative lot detected VANGL2 immunoreactivity co-localized with that of VANGL1 at the cell membrane of the stereociliary hair bundles by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed cochleae whole mount sections. A drastically reduced VANGL2 was observed in tissue sections from homozygous Vangl2 Looptail mutant (Lp/Lp) mice (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
Immunoprecipitation Analysis: A representative lot immunoprecipitated VANGL2, but not VANGL1, N-terminal fragment GST fusion, as well as endogenous VANGL2 from untreated, but not VANGL2 shRNA-treated SK-BR-7 human breast carcinoma cells (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
Western Blotting Analysis: A representative lot detected the endogenous VANGL2 as well as exogenously expressed yellow fluorescent protein Venus-tagged VANGL2 in transfected in MEC1 human chronic lymphocytic leukemia (CLL) cells (Kaucká, M., et al. (2015). Cell Commun Signal. 13:2).
Western Blotting Analysis: A representative lot detected VANGL2, but not VANGL1, N-terminal fragment GST fusion, as well as endogenous VANGL2 in lysate from untreated, but not VANGL2 shRNA-treated SK-BR-7 human breast carcinoma cells (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
Western Blotting Analysis: A representative lot detected higher VANGL2 expression in mouse brain and lung than kidney. A decreased cochlear VANGL2 expression was found among mice with heterozygous Vangl2 Looptail mutation (Lp), the decrease was even more pronounced among homozygous Vangl2(Lp/Lp) mice (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
Immunofluorescence Analysis: A representative lot detected VANGL2 immunoreactivity co-localized with that of VANGL1 at the cell membrane of the stereociliary hair bundles by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed cochleae whole mount sections. A drastically reduced VANGL2 was observed in tissue sections from homozygous Vangl2 Looptail mutant (Lp/Lp) mice (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
Immunoprecipitation Analysis: A representative lot immunoprecipitated VANGL2, but not VANGL1, N-terminal fragment GST fusion, as well as endogenous VANGL2 from untreated, but not VANGL2 shRNA-treated SK-BR-7 human breast carcinoma cells (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
Western Blotting Analysis: A representative lot detected the endogenous VANGL2 as well as exogenously expressed yellow fluorescent protein Venus-tagged VANGL2 in transfected in MEC1 human chronic lymphocytic leukemia (CLL) cells (Kaucká, M., et al. (2015). Cell Commun Signal. 13:2).
Western Blotting Analysis: A representative lot detected VANGL2, but not VANGL1, N-terminal fragment GST fusion, as well as endogenous VANGL2 in lysate from untreated, but not VANGL2 shRNA-treated SK-BR-7 human breast carcinoma cells (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
Western Blotting Analysis: A representative lot detected higher VANGL2 expression in mouse brain and lung than kidney. A decreased cochlear VANGL2 expression was found among mice with heterozygous Vangl2 Looptail mutation (Lp), the decrease was even more pronounced among homozygous Vangl2(Lp/Lp) mice (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
Research Category
Neuroscience
Neuroscience
Jakość
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected VANGL2 in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected VANGL2 in 10 µg of mouse brain tissue lysate.
Opis wartości docelowych
~60 kDa observed. 59.71 kDa (human) and 59.77 kDa (mouse & rat) calculated. Uncharacterized bands may be observed in some lysate(s).
Postać fizyczna
Format: Purified
Protein G purified.
Purified rat IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Inne uwagi
Concentration: Please refer to lot specific datasheet.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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