MABF1968

Anti-Microglobulin beta2 Antibody, clone BBM.1

clone BBM.1, from mouse

• Synonim(y): B2M
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: BBM.1, monoclonal
• reaktywność gatunkowa: human
• opakowanie: antibody small pack of 25 μg
• metody: flow cytometry: suitable; immunohistochemistry: suitable (paraffin); inhibition assay: suitable; western blot: suitable
• izotyp: IgG2bκ
• numer dostępu NCBI: NP_004039
• numer dostępu UniProt: P61769
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... B2M(567)

Opis

Opis ogólny

Beta-2-microglobulin (UniProt: P61769) is encoded by the B2M (also known as CDABP0092, HDCMA22P) gene (Gene ID: 567) in human. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC) that is involved in the presentation of peptide antigens to the immune system. It is synthesized with a signal peptide of 20 amino acids, which is subsequently cleaved to generate the mature form. It also contains an Ig-like C1 type domain (aa 25-113). Beta-2-microglobulin lacks a transmembrane anchoring domain and is found in secreted as well as in complex. Serum beta-2-microglobulin levels are a reflection of cell turnover and its levels are shown to be elevated in fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin′s lymphoma. Its levels may be elevated either due to higher rates of synthesis as observed in HIV infection and malignancy or due to impaired renal filtration due to renal insufficiency.

Specyficzność

Clone BBM.1 detects Beta-2-microglobulin in human.

Immunogen

MOLT-4, a Human acute lymphoblastic leukemia cell line.

Zastosowanie

Anti-Microglobulin beta2, clone BBM.1, Cat. No. MABF1968, is a mouse monoclonal antibody that detects Microglobulin beta2 and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition assays, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected Microglobulin beta2 in Immunohistochemistry applications (Josson, S., et. al. (2011). Cancer Res. 71(7):2600-10).

Inhibition Analysis: A representative lot inhibited Microglobulin beta2 activity (Parham, P., et. al. (1983). J Biol Chem. 258(10):6179-86).

Flow Cytometry Analysis: A representative lot detected Microglobulin beta2 in Flow Cytometry applications (Drukker, M., et. al. (2012). Nat Biotechnol. 30(6):531-42).

Immunohistochemistry Analysis: A 1:50-1:1,000 dilution from a representative lot detected Microglobulin beta2 in human breast cancer and human tonsil tissues.

Research Category
Inflammation & Immunology

Jakość

Evaluated by Western Blotting in MOLT-4 cell lysates

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Microglobulin beta2 in 10 µg of MOLT-4 cell lysate.

Opis wartości docelowych

~14 kDa observed; 13.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1