MABE1805
Anti-Rabex-5 Antibody, clone G32
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Informacje o tej pozycji
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
G32, monoclonal
Application:
WB
Citations:
-
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
G32, monoclonal
mol wt
calculated mol wt 56.89 kDa, observed mol wt ~57 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
western blot: suitable
isotype
IgG2aκ
epitope sequence
N-terminal
Protein ID accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... RABGEF1(2742)
General description
Rab5 GDP/GTP exchange factor (UniProt: Q9UJ41; also known as RAP1, Rabaptin-5-associated exchange factor for Rab5, Rabex-5) is encoded by the RABGEF1 (also known as RABEX5) gene (Gene ID: 27342) in human. Rabex-5 is a guanine nucleotide exchange factor that catalyzes the exchange of GTP for GDP on the Rab5 subfamily of low molecular weight GTPases. It forms a tight complex with Rabaptin-5 that augments its exchange activity and generates GTP-bound, membrane-associated Rab5, which in turn binds Rabaptin-5 and stabilizes the Rabex-5- Rabaptin-5 complex on endosomes. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. It can also act as a ubiquitin ligase. It binds to ubiquitin (Ub) through two independent ubiquitin-binding domains (UBDs). These UBDs determine a number of properties of Rabex-5, including its coupled monoubiquitination and interaction in vivo with ubiquitinated epidermal growth factor receptors. The first Ub binding region is localized in amino acids 2-49 and is named as RUZ domain (Rabex-5 ubiquitin binding zinc finger). The second Ub binding region is highly conserved and is localized in amino acids 48-74. In this domain Ala58 is shown to be critical for Ub binding and A58G mutagenesis causes significant reduction in its binding to Ub. (Ref.; Penengo, L., et al. (2006). Cell. 124. 1183-1195; Mattera, R., et al. (2006). J. Biol. Chem. 281(10); 6874-6883).
Immunogen
GST-tagged full length recombinant human Rabex-5.
Application
Quality Control Testing
Evaluated by Western Blotting in lysates from HeLa cells.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Rabex-5 in lysates from HeLa cells transfected with either Rabex-5 siRNA or Lamin siRNA. Higher expression was detected in Lamin siRNA transfected HeLa cells.
Tested Applications
Western Blotting Analysis: A representative lot detected Rabex-5 in Western Blotting applications (Penengo, L., et al. (2006). Cell. 124(6): 1183-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysates from HeLa cells.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Rabex-5 in lysates from HeLa cells transfected with either Rabex-5 siRNA or Lamin siRNA. Higher expression was detected in Lamin siRNA transfected HeLa cells.
Tested Applications
Western Blotting Analysis: A representative lot detected Rabex-5 in Western Blotting applications (Penengo, L., et al. (2006). Cell. 124(6): 1183-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Rabex-5, clone G32, Cat. No. MABE1805, is a mouse monoclonal antibody that detects Rebex-5 and is tested for use in Western Blotting.
Biochem/physiol Actions
Clone G32 is a mouse monoclonal antibody that detects Rabex-5. It targets an epitope within 49 amino acids from the N-terminal region.
Physical form
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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