About This Item
Kod UNSPSC:
12352203
NACRES:
NA.41
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
G32, monoclonal
masa cząsteczkowa
calculated mol wt 56.89 kDa
observed mol wt ~57 kDa
oczyszczone przez
using protein G
reaktywność gatunkowa
human
opakowanie
antibody small pack of 100 μg
metody
western blot: suitable
izotyp
IgG2aκ
sekwencja epitopowa
N-terminal
numer dostępu Protein ID
numer dostępu UniProt
Warunki transportu
ambient
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... RABGEF1(2742)
Opis ogólny
Rab5 GDP/GTP exchange factor (UniProt: Q9UJ41; also known as RAP1, Rabaptin-5-associated exchange factor for Rab5, Rabex-5) is encoded by the RABGEF1 (also known as RABEX5) gene (Gene ID: 27342) in human. Rabex-5 is a guanine nucleotide exchange factor that catalyzes the exchange of GTP for GDP on the Rab5 subfamily of low molecular weight GTPases. It forms a tight complex with Rabaptin-5 that augments its exchange activity and generates GTP-bound, membrane-associated Rab5, which in turn binds Rabaptin-5 and stabilizes the Rabex-5- Rabaptin-5 complex on endosomes. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. It can also act as a ubiquitin ligase. It binds to ubiquitin (Ub) through two independent ubiquitin-binding domains (UBDs). These UBDs determine a number of properties of Rabex-5, including its coupled monoubiquitination and interaction in vivo with ubiquitinated epidermal growth factor receptors. The first Ub binding region is localized in amino acids 2-49 and is named as RUZ domain (Rabex-5 ubiquitin binding zinc finger). The second Ub binding region is highly conserved and is localized in amino acids 48-74. In this domain Ala58 is shown to be critical for Ub binding and A58G mutagenesis causes significant reduction in its binding to Ub. (Ref.; Penengo, L., et al. (2006). Cell. 124. 1183-1195; Mattera, R., et al. (2006). J. Biol. Chem. 281(10); 6874-6883).
Specyficzność
Clone G32 is a mouse monoclonal antibody that detects Rabex-5. It targets an epitope within 49 amino acids from the N-terminal region.
Immunogen
GST-tagged full length recombinant human Rabex-5.
Zastosowanie
Quality Control Testing
Evaluated by Western Blotting in lysates from HeLa cells.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Rabex-5 in lysates from HeLa cells transfected with either Rabex-5 siRNA or Lamin siRNA. Higher expression was detected in Lamin siRNA transfected HeLa cells.
Tested Applications
Western Blotting Analysis: A representative lot detected Rabex-5 in Western Blotting applications (Penengo, L., et al. (2006). Cell. 124(6): 1183-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysates from HeLa cells.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Rabex-5 in lysates from HeLa cells transfected with either Rabex-5 siRNA or Lamin siRNA. Higher expression was detected in Lamin siRNA transfected HeLa cells.
Tested Applications
Western Blotting Analysis: A representative lot detected Rabex-5 in Western Blotting applications (Penengo, L., et al. (2006). Cell. 124(6): 1183-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Rabex-5, clone G32, Cat. No. MABE1805, is a mouse monoclonal antibody that detects Rebex-5 and is tested for use in Western Blotting.
Postać fizyczna
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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