MAB5256A5

Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor^™ 555 Conjugate

clone NE14, from mouse, ALEXA FLUOR^™ 555

• Synonim(y): Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: ALEXA FLUOR^™ 555
• Clone: NE14, monoclonal
• Application: ICC, IHC
• Citations: 6

Właściwości

• biological source: mouse
• Quality Segment: 100
• conjugate: ALEXA FLUOR^™ 555
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: NE14, monoclonal
• species reactivity: mouse, rat, pig
• species reactivity (predicted by homology): human (100% sequence homology), porcine (immunogen homology)
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable
• isotype: IgG1
• NCBI accession no.: NP_066554
• UniProt accession no.: P12036
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NEFH(4744) ; mouse ... Nefh(380684) ; pig ... Nefh(100156492) ; rat ... Nefh(24587)

Opis

General description

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information and epitope maps, search "neurofilament" on our website.

Application

Detect Neurofilament 200kDa using this Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor 555 Conjugate validated for use in ICC & IHC.

Analysis Note

Evaluated by Immunocytochemistry in rat E18 primary cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neurofilament in rat E18 primary cortex cells.

Other Notes

Unconjugated Anti-Neurofilament has been shown to work with multiple species including Human and Porcine.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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