Przejdź do zawartości
Merck

MAB3580

Anti-Green Fluorescent Protein Antibody

Chemicon®, from mouse

Synonim(y):

GFP, eGFP

Slide 1 of 5
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.

Informacje o tej pozycji

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
monoclonal
Application:
ELISA, ICC, IHC, WB
Citations:
206

Kluczowe dokumenty

Wyświetl całą dokumentację

Przejdź do

Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomóc

biological source

mouse

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1

UniProt accession no.

P42212

target post-translational modification

unmodified

Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów

Porównaj podobne pozycje

Wyświetl pełne porównanie

Pokaż różnice

1 of 4

Ta pozycja
SAB4200681G6795AB3080
Anti-Green Fluorescent Protein Antibody Chemicon®, from mouse

Sigma-Aldrich

MAB3580

Anti-Green Fluorescent Protein Antibody

Anti-Green Fluorescent Protein (GFP) antibody, Mouse monoclonal clone GFP-20, purified from hybridoma cell culture

Sigma-Aldrich

SAB4200681

Anti-Green Fluorescent Protein (GFP) antibody, Mouse monoclonal

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal clone GSN24, purified from hybridoma cell culture

Sigma-Aldrich

G6795

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal

Anti-Green Fluorescent Protein Antibody Chemicon®, from rabbit

Sigma-Aldrich

AB3080

Anti-Green Fluorescent Protein Antibody

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

clone

monoclonal

clone

GFP-20, monoclonal

clone

GSN24, monoclonal

clone

polyclonal

antibody form

affinity purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

UniProt accession no.

P42212

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

P42212

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

immunoblotting: 1-2 μg/mL using whole extract of human HEK-293T cells over-expressing GFP tagged fusion protein., immunoprecipitation (IP): 5-10 μg using whole extract of human HEK-293T cells over-expressing GFP tagged fusion protein.

technique(s)

western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

General description

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
MW for GFP is 27 kDa Dependent upon the molecular weight of the fusion protein being detected.

Immunogen

GFP-BSA

Application

Detect Green Fluorescent Protein using this Anti-Green Fluorescent Protein Antibody validated for use in ELISA, IC, IH & WB.
Western blot: 1:2,500-1:5,000. Recommended dilution buffer is PBS containing 1 mg/mL BSA.

Immunocytochemistry: 1:500-1:1,000

Immunohistochemistry: 1:500-1:1,000 on paraformaldehyde fixed tissues with an overnight incubation using Nickel enhanced DAB for detection. Suggested permeabilization method is 0.5% Triton X-100. Recommended blocking buffer is PBS containing BSA and serum from the host of the secondary antibody. Recommended dilution buffer is PBS containing 1 mg/mL of BSA.

ELISA: 1:1,000-1:2,000 in a direct assay.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Reacts with Green Fluorescent Protein (GFP). The antibody reacts with various forms of GFP including eGFP including those found in vectors supplied by Clonetech and Invitrogen. There is not cross-reactivity to RFP. YFP has not been tested. This antibody works well for immunohistochemistry.

Physical form

Format: Purified
Liquid in PBS containing 1 mg/mL BSA. Contains no preservative.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Ta strona może zawierać tekst przetłumaczony maszynowo.

Nie możesz znaleźć właściwego produktu?  

Wypróbuj nasz Narzędzie selektora produktów.

Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 2

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Multiple phenotypes resulting from a mutagenesis screen for pharynx muscle mutations in Caenorhabditis elegans.
Ferrier, A; Charron, A; Sadozai, Y; Switaj, L; Szutenbach, A; Smith, PA
Testing null
Drosophila Dpp morphogen movement is independent of dynamin-mediated endocytosis but regulated by the glypican members of heparan sulfate proteoglycans.
Belenkaya, TY; Han, C; Yan, D; Opoka, RJ; Khodoun, M; Liu, H; Lin, X
Cell null
Magdalena Jasińska et al.
Molecular neurobiology, 53(7), 4701-4712 (2015-09-01)
Mir-132 is a neuronal activity-regulated microRNA that controls the morphology of dendritic spines and neuronal transmission. Similar activities have recently been attributed to matrix metalloproteinase-9 (MMP-9), an extrasynaptic protease. In the present study, we provide evidence that miR-132 directly regulates
Benjamin J Spink et al.
Nature structural & molecular biology, 15(6), 591-597 (2008-05-31)
Myosin VI has challenged the lever arm hypothesis of myosin movement because of its ability to take approximately 36-nm steps along actin with a canonical lever arm that seems to be too short to allow such large steps. Here we
Elsa Suberbielle et al.
Nature communications, 6, 8897-8897 (2015-12-01)
Maintaining DNA integrity is vital for all cells and organisms. Defective DNA repair may contribute to neurological disorders, including Alzheimer's disease (AD). We found reduced levels of BRCA1, but not of other DNA repair factors, in the brains of AD
Wyświetl wszystkie powiązane dokumenty

Numer pozycji handlu globalnego

SKUNUMER GTIN
MAB358004053252579530

Questions

Reviews

No rating value

Active Filters

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej