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MAB345A4

Sigma-Aldrich

Anti-O4 Antibody, clone 81, Alexa Fluor488 Conjugate

clone 81 (mAB 04), from mouse, ALEXA FLUOR 488

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

białko sprzężone

ALEXA FLUOR 488

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

81 (mAB 04), monoclonal

reaktywność gatunkowa

rat

reaktywność gatunkowa (przewidywana na podstawie homologii)

human (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), mouse (based on 100% sequence homology)

metody

immunocytochemistry: suitable

izotyp

IgM

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

Opis ogólny

Oligodendrocytes and astrocytes are derived from common precursor cells, glioblasts (Sommer, 1981). O-Antigens are sulfatides, which function as differentiation markers on the surface of oligodendrocytes of the central nervous system. O4 is formed postnatally (Schachner, 1981) and is a marker for cell bodies and processes of oligodendrocytes types I and II (hairy eyeball type). During oligodendrocyte differentiation, O4 occurs in pro-oligodendrocytes, but not in O-2A-progenitor cells. O4 occurs from day 3 onwards in cell cultures of embryonic mouse brain. Anti-O4 can be used in myelination, demyelination and remyelination studies and in regeneration experiments.

Specyficzność

This antibody recognizes Oligodendrocyte marker O4. Also reacts with certain galactolipids in sperm.

Immunogen

Homogenate of white matter of corpus callosum from bovine brain.

Zastosowanie

Anti-O4 Antibody, clone 81, Alexa Fluor488 Conjugate detects level of O4 & has been published & validated for use in ICC.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Jakość

Evaluated by Immunocytochemistry in undifferentiated rat oligodendrocyte precursor cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected O4 in undifferentiated rat oligodendrocyte precursor cells.

Postać fizyczna

Precipitation followed by ion exchange purification
Purified mouse monoclonal IgM conjugated to Alexa Fluor 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Przechowywanie i stabilność

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Komentarz do analizy

Control
Undifferentiated rat oligodendrocyte precursor cells

Informacje prawne

ALEXA FLUOR is a trademark of Life Technologies

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Jo Anne Stratton et al.
Cell reports, 24(10), 2561-2572 (2018-09-06)
Pro-regenerative macrophages are well known for their role in promoting tissue repair; however, their specific roles in promoting regeneration of the injured nerve are not well defined. Specifically, how macrophages interact with Schwann cells following injury during remyelination has been
Ajantha Abey et al.
Brain pathology (Zurich, Switzerland), 31(1), 144-162 (2020-08-19)
Some aged community dogs acquire a degenerative syndrome termed Canine Cognitive Dysfunction (CCD) that resembles human dementia because of Alzheimer's Disease (AD), with comparable cognitive and behavioral deficits. Dogs also have similar neuroanatomy, share our domestic environment and develop amyloid-β

Produkty

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

Uzyskanie i scharakteryzowanie funkcjonalnych komórek progenitorowych oligodendrocytów (OPC) pochodzących z ludzkich neuronalnych komórek macierzystych, które skutecznie mielinizują pierwotne neurony w hodowli.

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