MAB3230

Anti-Cytokeratin 10 Antibody, clone RKSE60

clone RKSE60, Chemicon^®, from mouse

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: RKSE60, monoclonal
• Application: FACS, ICC, IHC, WB
• Citations: 11

Właściwości

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RKSE60, monoclonal
• species reactivity: rat, canine, human, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_000421.2
• UniProt accession no.: P13645
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... KRT10(3858)

Opis

General description

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.

Immunogen

Cytoskeletal preparation from human squamous keratinizing epithelium.

Application

Anti-Cytokeratin 10 Antibody, clone RKSE60 detects level of Cytokeratin 10 & has been published & validated for use in FC, WB, IC, IH.

Research Category
Cell Structure

Research Sub Category
Cytokeratins

Western blot

Immunohistochemistry on frozen tissue sections.

Immunocytochemistry

Flow cytometry

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Cytokeratin 10. MAB3230 reacts with keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas. For the recognition of keratinizing cells in squamous cell carcinomas from epidermis, lung, bladder, cervix, esophagus etc. The antibody reacts only with suprabasal cells of the epidermis and not with basal cells.

Physical form

Format: Purified

Liquid in buffer with 0.1% sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 2