MAB1430
Anti-Collagen Type IV Antibody, clone 24.12.8 (PHM-12)
clone 24.12.8 (PHM-12), Chemicon®, from mouse
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Informacje o tej pozycji
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
24.12.8 (PHM-12), monoclonal
Application:
IHC (p)
Citations:
17
Pomoc techniczna
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Pozwól nam pomócbiological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
clone
24.12.8 (PHM-12), monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
General description
BIOCHEMISTRY:
p I = 5.5
p I = 5.5
Immunogen
Human glomeruli
Application
Detect Collagen Type IV using this Anti-Collagen Type IV Antibody, clone 24.12.8 (PHM-12) validated for use in IH, IH(P).
The antibody is suitable for use on both frozen and protease digested paraffin processed tissue.
SUGGESTED USAGE
Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:40 will be applicable to most commonly used systems.
Optimal working dilutions must be determined by the end user.
SUGGESTED USAGE
Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:40 will be applicable to most commonly used systems.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
MAB1430 provides a marker for an antigen known to be important in glomerular cell-matrix interactions, and should prove useful in research into the mechanisms involved in renal pathology. This antibody reacts with glomerular and tubular basement membranes in kidney as well as basal lamina of capillaries. It also labels mesangial cells and matrix within the glomerulus as well as basement membrane structures in all organs examined (Gusterson et al., 1984).
Physical form
Format: Purified
Purified immunoglobulin presented as a liquid in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide.
Preparation Note
Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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