FCABS325PE

Milli-Mark^® Anti-acetyl-Histone H3-PE Antibody

Milli-Mark^®, from rabbit

• Synonim(y): H3t, H3/t, H3/g, Histone H3.1t
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: PE
• Clone: polyclonal
• Application: FACS
• Citations: 4

Właściwości

• biological source: rabbit
• Quality Segment: 100
• conjugate: PE
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: Tetrahymena sp., human, mouse
• manufacturer/tradename: Milli-Mark^®
• technique(s): flow cytometry: suitable
• isotype: IgG
• NCBI accession no.: NP_003484
• UniProt accession no.: Q16695
• shipped in: wet ice
• target post-translational modification: acetylation (Lys9,Lys14)
• Gene Information: human ... H3F3B(3021)

Opis

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.

15.5 kDa Calculated

Immunogen

KLH-conjugated peptide corresponding to amino acids 1-20 of Tetrahymena histone H3 (ARTKQTAR[K*]STGG[K*]APRKQLC) where K* is acetylated

Application

This Milli-Mark Anti-acetyl-Histone H3-PE Antibody is validated for use in FC for the detection of acetyl-Histone H3.

Biochem/physiol Actions

Antibody recognizes Tetrahymena acetyl Histone H3

This sequence is highly conserved and predicted to have broad species crossreactivity.

Physical form

Purified rabbit polyclonal IgG conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA

Analysis Note

Evaluated by flow cytometry using HeLa cells

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

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