ABS82-AF647

Anti-VEGF Antibody, Alexa Fluor^™ 647 Conjugate

from rabbit, ALEXA FLUOR^™ 647

• Synonim(y): Anti-MVCD1, Anti-VEGF, Anti-VPF, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, Alexa Fluor^™ 647 Conjugate
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• białko sprzężone: ALEXA FLUOR^™ 647
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• reaktywność gatunkowa: mouse, human, rat
• reaktywność gatunkowa (przewidywana na podstawie homologii): nonhuman primates (based on 100% sequence homology)
• metody: immunocytochemistry: suitable
• izotyp: IgG
• numer dostępu NCBI: NP_001020537
• numer dostępu UniProt: P15692
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... VEGFA(7422)

Opis

Opis ogólny

VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively). All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and also plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.

Specyficzność

Other homologies: Other homologies: Bovine (85% sequence homology). Rat (70% sequence homology). Mouse (65% sequence homology).

This antibody recognizes VEGF at the N-terminus.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to human VEGF at the N-terminus.

Zastosowanie

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VEGF in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. ABS82) is shown to be suitable also for Western blotting and immunohistochemistry (Paraffin) applications.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Jakość

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected VEGF in HeLa cells.

Opis wartości docelowych

~55 kDa observed

Postać fizyczna

Affinity purified

Purified rabbit antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Informacje prawne

ALEXA FLUOR is a trademark of Life Technologies

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable