ABE572

Anti-N6-methyladenosine (m6A) Antibody

from rabbit

• Synonim(y): m6A
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Technique(s): ChIP: suitable, RIP: suitable, dot blot: suitable
• Application: ChIP, DB, RIP
• Citations: 91

Właściwości

• biological source: rabbit
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity (predicted by homology): all
• technique(s): ChIP: suitable, RIP: suitable, dot blot: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Opis

General description

N6-methyladenosine (m6A), or methylation of the N6 position of adenosine is a posttranscriptional modification of RNA. Due to a lack of analytical methods, N6-methyladenosine is poorly understood, but new evidence suggests that it is a very common base modification and important physiological regulator. N6-methyladenosine is markedly increased throughout brain development, and is enriched near stop codons, microRNA-binding sites and UTRs, which indicates a fundamental role in the regulation of gene expression. N6-methyladenosine is also highly conserved between human and mouse.

Immunogen

BSA-conjugated N6-methyladenosine.

Application

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in RNA containing N6-methyladenosine (Dominissini, D., et al. (2012). Nature. 485(7397):201-206.; Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.).

(RNA) Immunoprecipitation Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in methylated RNA containing N6-methyladenosine (Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.).

Dot Blot Analysis: A representative lot from an independent laboratory detected N6-methyladenosine in a panel of modified oligonucleotides (Meyer, K. D., et al. (2012). Cell. 149(7):1635-1646.; Jia, G., et al. (2011). Nat Chem Biol. 7(12):885-887.).

Detect N6-methyladenosine (m6A) using Anti-N6-methyladenosine (m6A) Antibody validated for use in Dot Blot, Chromatin IP (ChIP) & RNA Binding Protein IP (RIP).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Dot Blot in oligomers containing N6-methyladenosine.

Dot Blot Analysis: 1 µg/mL of this antibody detected N6-methyladenosine in oligomers containing N6-methyladenosine.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Klasa składowania: 12 - Non Combustible Liquids