AB3274

Anti-Aquaporin 2 Antibody

Chemicon^®, from rabbit

• Synonim(y): AQP2, AQP-CD, WCD-CD
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: rat, human
• Application: IHC, WB
• Citations: 28

Właściwości

• biological source: rabbit
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_000486.3
• UniProt accession no.: P41181
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... AQP2(359)

Opis

Immunogen

Highly purified peptide corresponding to residues 254-271 of rat or mouse Aquaporin 2 (SwissProt accession number P34080).

Application

Anti-Aquaporin 2 Antibody is an antibody against Aquaporin 2 for use in WB, IH.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blotting: 1:200-1:1000 using ECL on rat kidney membranes.

Immunohistochemistry on rat kidney sections.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Aquaporin 2.

SPECIES REACTIVITIES: The epitope specific for Aquaporin 2 is identical in rat and mouse and only slightly different in human (17/18 amino acids). Reactivity with other species has not yet been tested.

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Preparation Note

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 0.5ug of purified peptide with 1 μg of antibody for one hour at room temperature.

Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3