AB1928
Anti-Integrin alpha 5 (ITGA5/CD49E) Antibody
CHEMICON®, rabbit polyclonal
Synonim(y):
CD49e, Fibronectin Receptor alpha Subunit
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Wybierz wielkość
100 μL
1940,00 zł
1940,00 zł
Przewidywany termin wysyłki22 kwietnia 2025Szczegóły
Rekombinowane, niezawierające konserwantów przeciwciało jest dostępne dla Twojego celu. Wypróbuj ZRB1122
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100 μL
1940,00 zł
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
1940,00 zł
Przewidywany termin wysyłki22 kwietnia 2025Szczegóły
Rekombinowane, niezawierające konserwantów przeciwciało jest dostępne dla Twojego celu. Wypróbuj ZRB1122
Poproś o zamówienie zbiorcze
Polecane produkty
Nazwa produktu
Anti-Integrin α5 Antibody, CT, Intracellular, serum, Chemicon®
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
serum
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
human, chicken, hamster, rat, mouse
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... ITGA5(3678)
Opis ogólny
Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.
Specyficzność
Antibody recognizes integrin alpha5. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. No cross-reactivity to alpha1, alpha2, alpha3, alpha4, alpha6 or alphaV. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus, intracellular.
Reactivity with other species has not been determined.
Immunogen
Synthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha5 integrin subunit.
Zastosowanie
Anti-Integrin α5 Antibody, C-terminus, Intracellular detects level of Integrin α5 & has been published & validated for use in ELISA, IC, IF, IH, IP, RIA & WB.
Western Blot Analysis:
1:1000 dilution of a previous lot was used.
ELISA:
1:500-1:2000 dilution of a previous lot was used in ELISA.
Immunoprecipitation:
Recommended use of 5 μL of antibody for 5x106 cells.
Immunohistochemistry:
1:1000 dilution of a previous lot was used in tissue staining; suggested for use on acetone fixed tissue only.
Immunocytochemistry:
1:1000 of a previous lot was used in immunocytochemistry.
Optimal working dilutions must be determined by the end user.
1:1000 dilution of a previous lot was used.
ELISA:
1:500-1:2000 dilution of a previous lot was used in ELISA.
Immunoprecipitation:
Recommended use of 5 μL of antibody for 5x106 cells.
Immunohistochemistry:
1:1000 dilution of a previous lot was used in tissue staining; suggested for use on acetone fixed tissue only.
Immunocytochemistry:
1:1000 of a previous lot was used in immunocytochemistry.
Optimal working dilutions must be determined by the end user.
Jakość
Routinely evaluated by Western Blot on HUVEC lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected integrin alpha 5 on 10 μg of HUVEC lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected integrin alpha 5 on 10 μg of HUVEC lysates.
Opis wartości docelowych
114 kDa
Postać fizyczna
Rabbit polyclonal antiserum in buffer containing 0.05% sodium azide.
Komentarz do analizy
Control
Mouse 3T3 fibroblasts Skin (Basement Membrane).
Mouse 3T3 fibroblasts Skin (Basement Membrane).
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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A B McGeachie et al.
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