71350-M

Rosetta-gami 2 Competent Cells - Novagen

Escherichia coli, rod shaped

• Kod UNSPSC: 41106202
• NACRES: NA.81

Właściwości

• product name: Rosetta-gami 2 Competent Cells - Novagen, Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
• pochodzenie biologiczne: Escherichia coli
• Poziom jakości: 100
• producent / nazwa handlowa: Novagen^®
• warunki przechowywania: OK to freeze
• tryb wzrostu: adherent or suspension
• morfologia: rod shaped
• metody: microbiological culture: suitable
• transformacja komórek: transformation efficiency: 2×10⁶ cfu/μg
• Warunki transportu: dry ice
• temp. przechowywania: −70°C

Opis

Opis ogólny

Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac⁺ lacI^q pro] gor522::Tn10 trxB pRARE2 (Cam^R, Str^R, Tet^R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Rosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E. coli. These trxB/gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs.

Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.

Komponenty

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta-gami 2 Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

Ostrzeżenie

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Informacje prawne

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany