612200-M

Tissue Plasminogen Activator, Human

• Synonim(y): tPA
• UNSPSC Code: 12352202
• NACRES: NA.25
• Form: lyophilized
• Assay: ≥95% (SDS-PAGE)

Właściwości

• assay: ≥95% (SDS-PAGE)
• Quality Segment: 100
• form: lyophilized
• specific activity: ≥300 KU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• storage temp.: −20°C

Opis

General description

Native tissue plasminogen activator from CHO cells. A serine protease that contains a single chain of 527 amino acid residues. Binds to fibrin via lysine binding sites at its amino terminus and activates bound plasminogen. Cleaves plasminogen to form active plasmin, the proteolytic enzyme that dissolves clots. Reported to be involved in neurite outgrowth, regeneration, and migration. Plays an important role in excitotoxin-induced neuronal degeneration. Dihydropyridine Ca²⁺ antagonist may increase levels of tPA.

Physical form

Lyophilized from 100 mM PBS, 3.5 mg/ml L-arginine, 0.001% Tween^®-80 Detergent.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Reconstitute with diH₂O to a final concentration of 1 mg/ml (e.g., 100 µg should be reconstituted in 100 µl).

Other Notes

Kim, Y.H., et al. 1999. Science284, 647.
Tsirka, S.E., et al. 1995. Nature 377, 340.
Winther, K., et al. 1992. J. Cardiovasc. Pharmacol.19, S21.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable