58346-M

Tetracycline, Hydrochloride

CAS 64-17-5 is a broad-based antibiotic agent that blocks protein synthesis by inhibiting binding of aminoacyl tRNA to the A-site of ribosomes. Induces cold shock-response and enhances P450 expression in bacteria.

• Synonim(y): Tetracycline, Hydrochloride
• Wzór empiryczny (zapis Hilla): C₂₂H₂₄N₂O₈
• Numer CAS: 64-75-5
• Masa cząsteczkowa: 444.43
• Numer MDL: MFCD00078142
• Kod UNSPSC: 51101500

Właściwości

• Poziom jakości: 200
• Postać: crystalline solid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; desiccated; protect from light
• stężenie: ≥900 μg/mg
• kolor: yellow
• rozpuszczalność: water: 20 mg/mL; ethanol: 5 mg/mL
• temp. przechowywania: 10-30°C
• InChI: 1S/C22H24N2O8.ClH/c1-21(31)8-5-4-6-11(25)12(8)16(26)13-9(21)7-10-15(24(2)3)17(27)14(20(23)30)19(29)22(10,32)18(13)28;/h4-6,9-10,15,25-26,30-32H,7,23H2,1-3H3;1H/b20-14-;/t9-,10-,15-,21+,22-;/m0./s1
• Klucz InChI: DYQBMRZOOXYHIM-NCKOGSTASA-N

Opis

Opis ogólny

An antibiotic that inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit, preventing binding of aminoacyl tRNA to the A-site and blocking translocation. Used commonly for selection of the bacterial tet^r gene whose product, a 399-amino acid membrane-associated protein, functions in active export of the drug from the cell.

Broad-based antibiotic agent that blocks protein synthesis by inhibiting binding of aminoacyl tRNA to the A-site of ribosomes. Induces cold shock-response and enhances P450 expression in bacteria.

Działania biochem./fizjol.

Primary Target
binding of aminoacyl tRNA to the A-site of ribosomes

Ostrzeżenie

Toxicity: Standard Handling (A)

Rekonstytucja

Following reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for 1 month at -20°C. Agar plates are stable for 2 weeks at 4°C. Autoclaved media should be cooled to ≤55°C before adding tetracycline.

Inne uwagi

Kusano, K., et al. 1999. Arch. Biochem. Biophys. 367, 129.
Lech, K. and Brent, R. 1995. In Current Protocols in Molecular Biology, Vol. 1 (Ausubel, F.M., et al. eds.) John Wiley & Sons, New York Unit 1.4.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York, p. 1.6, A.6.
Chopra, I. and Howe, T.G.B. 1978. Microbiol. Rev. 42, 707.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07,GHS09
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H315,H319,H335,H361fd,H410
• Zwroty wskazujące środki ostrożności: P202 - P261 - P273 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Klasyfikacja zagrożeń: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable