The concentration is 50 µg/ml in PBS, pH 7.4.
537060
Propidium Iodide Solution
A convenient form of propidium iodide useful for flow cytometry studies.
Synonim(y):
Propidium Iodide Solution
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
5 mL | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 790,00 zł |
Informacje o tej pozycji
Wzór empiryczny (zapis Hilla):
C27H34I2N4
Numer CAS:
Masa cząsteczkowa:
668.39
UNSPSC Code:
12352200
NACRES:
NA.47
MDL number:
Form:
liquid
Storage condition:
OK to freeze, protect from light
Pomoc techniczna
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Pozwól nam pomócQuality Segment
description
RTECS - SF7949600
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
input
sample type intact cells
fluorescence
λex 536 nm, λem 617 nm
shipped in
ambient
storage temp.
2-8°C
SMILES string
[I-].I.[N+](CCCN1C2=CC(=N)C=CC2=C3C(=C1c4ccccc4)C=C(C=C3)N)(CC)(CC)C
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
General description
Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Membrane impermeable DNA intercalator
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no
Physical form
50 µg/ml in PBS, pH 7.4.
Preparation Note
Following initial use, aliquot and freeze (-20°C).
Other Notes
Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Ta pozycja | |||
|---|---|---|---|
| form liquid | form solid | form liquid | form liquid |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename - |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| storage condition OK to freeze, protect from light | storage condition OK to freeze, protect from light | storage condition - | storage condition - |
| shipped in ambient | shipped in ambient | shipped in - | shipped in - |
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Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certyfikaty analizy (CoA)
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what is the concentration of the PI solution
1 answer-
Helpful?
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What is the recommended protocol for use/ working concentration?
1 answer-
The working concentration must be determined by the end user.
In the following reference: Cytometry. 1994 Sep 1;17(1):59-65. PMID: 7528124 DOI: 10.1002/cyto.990170108; 5ug/ML of PI was directly added in the culture medium for 30min. at 37C.Helpful?
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