345811

Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli

Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli, hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures.

• Numer WE: 3.2.1.103 (BRENDA, IUBMB)
• NACRES: NA.54
• UNSPSC Code: 12352202
• Form: liquid
• Recombinant: expressed in E. coli

Właściwości

• recombinant: expressed in E. coli
• Quality Segment: 100
• conjugate: (Glucosaminoglycan)
• form: liquid
• specific activity: ≥14 U/mL, ≥140 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: ambient
• storage temp.: 2-8°C

Opis

General description

Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.

Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8.

Physical form

Sterile-filtered in 20 mM Tris-HCl, pH 7.5.

Preparation Note

There is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.

Other Notes

Murata, T., et al. 2003. Eur. J. Biochem.270, 3709.
Scudder, P., et al. 1986. Eur. J. Biochem.157, 365.
Scudder, P., et al. 1984. J. Biol. Chem.259, 6586.
Scudder, P., et al. 1983. Biochem. J.213, 485.

One unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable