341595

FGF-2, Basic, Human, Recombinant, E. coli

• Synonim(y): FGF-2, Basic, Human, Recombinant, E. coli, rhbFGF, Fibroblast Growth Factor, Basic, Human, Recombinant, E. coli
• Numer CAS: 106096-93-9
• UNSPSC Code: 12352202
• MDL number: MFCD00081382

Właściwości

• assay: ≥97% (SDS-PAGE)
• Quality Segment: 100
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤1 EU/μg Endotoxin (EU/μg FGF)
• shipped in: ambient
• storage temp.: −70°C

Opis

General description

Recombinant, human basic fibroblast growth factor expressed in E. coli. A member of the FGF family of mitogenic peptides, comprised of seven related proteins showing 35-55% amino acid conservation within the family and 55% similarity with acidic FGF. Found in neuronal tissue (hippocampus, brain stem, peripheral ganglia, and the isocortex), pituitary, adrenal cortex, corpus luteum, and placenta. Some of the biological activities attributed to basic FGF include: mitogenic activity in vitro for cells of meso- and neuroectodermal origin (i.e. fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, bovine epithelial lens cells, osteoblasts, smooth muscle cells, and melanocytes); role in wound healing; induction of neuron differentiation, survival, and regeneration; chemoattractant effect and mitogenic activity in vitro for endothelial cells and angiogenic activity in vivo; role in tumor neovascularization. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits iNOS in bovine retinal pigmented epithelial cells. Binding of FGF to heparin or cell surface heparin sulfate proteoglycans is necessary for FGF binding to its high affinity receptors. Basic and acidic FGF appear to bind to the same high affinity receptors and show a similar range of biological activities.

Recombinant, human basic fibroblast growth factor expressed in E. coli. Potent mitogen for bone cells. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits inducible NOS in bovine retinal pigmented epithelial cells.

Biochem/physiol Actions

ED₅₀ = 100-250 pg/ml as monitored in a mitogenic assay by measuring FGF basic-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts

Physical form

Lyophilized from sterile-filtered 20 mM Tris-HCl, 1 M NaCl, 50 µg BSA/1 µg cytokine, pH 7.0.

Preparation Note

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 6 months at -70°C.

Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing 1 mM DTT and ≥0.1% HSA or BSA.

Other Notes

Goureau, O., et al. 1995. Eur. J. Biochem. 230, 1046.
Gibran, N.S., et al. 1994. J. Surg. Res. 56, 226.
Li, J.J., et al. 1993. Cancer72, 2253.
Mignatti, P., et al. 1992. J. Cell Physiol.151, 81.
Mignatti, P., et al. 1991. Proc. Natl. Acad. Sci. USA88, 11007.
Baird, A., and Klagsbrun, M. 1991. Cancer Cells3, 239.
Gospodarowicz, D. 1991. Ann. NY Acad. Sci.638, 1.
Basilico, C., et al. 1989. Ann. NY Acad. Sci.567, 95.
Burgess, W.H., and Maciag, T. 1989. Annu. Rev. Biochem.58, 575.
Rogelj, S., et al. 1989. J. Cell. Biochem.39, 13.
Rizzino, A., et al. 1988. Cancer Res.48, 4266.
Abraham, J.A., et al. 1986. EMBO J.5, 2523.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3